RGD Reference Report - Addition of MCP-1 and MIP-3β to the IL-8 appraisal in peritoneal fluid enhances the probability of identifying women with endometriosis. - Rat Genome Database

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Addition of MCP-1 and MIP-3β to the IL-8 appraisal in peritoneal fluid enhances the probability of identifying women with endometriosis.

Authors: Borrelli, G M  Kaufmann, A M  Abrão, M S  Mechsner, S 
Citation: Borrelli GM, etal., J Reprod Immunol. 2015 Jun;109:66-73. doi: 10.1016/j.jri.2015.01.003. Epub 2015 Feb 7.
RGD ID: 150521547
Pubmed: PMID:25704572   (View Abstract at PubMed)
DOI: DOI:10.1016/j.jri.2015.01.003   (Journal Full-text)

Chemokines have been associated with endometriosis. Our study was aimed at evaluating the levels of six chemokines--CXCL8 (IL-8), CXCL12 (SDF-1), CCL2 (MCP-1), CCL5 (RANTES), CCL19 (MIP-3β), and CCL21 (6-Ckine)--in the peritoneal fluid (PF) of patients with and controls without endometriosis by multiplexed cytokine assay. In this retrospective case-control study conducted at the Charité University Hospital, patients (n = 36) and controls (n = 27) were enrolled. The patients were separated into groups according to stage of the disease: I-II (n = 21), III-IV (n = 1 5), and according to clinical findings: peritoneal endometriosis (PE; n = 7), deep-infiltrating endometriosis (DIE) affecting the retrocervical area (n = 13) or the bowel/rectovaginal site (n = 14). The subjects were also separated according to the cycle phase: follicular (n = 14) or luteal (n = 8) and the previous use (n = 25) or not (n = 38) of hormones. PF was collected from all subjects (n = 63) consecutively during laparoscopy. The concentration of chemokines in the PF was assessed using Luminex(®) x-MAP(®) technology. Sensitivity and specificity were calculated. A model of multiple logistic regressions estimated the odds of endometriosis for each combination of the chemokines detected. We observed significantly higher concentrations of IL-8 (p < 0.001), MCP-1 (p = 0.014), and MIP-3β (p = 0.022) in the PF of women with endometriosis than in the controls. A joint evaluation revealed that elevated levels of the three chemokines had a positive endometriosis prediction value of 89.1%. The combined assessment of MCP-1, MIP-3β, and IL-8 concentration in PF improved the likelihood of identifying patients with endometriosis. Future studies should investigate this panel in peripheral blood samples.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CXCL8Humanendometriosis  IEP protein:increased expression:peritoneal fluid (human)RGD 
Cxcl15Mouseendometriosis  ISOCXCL8 (Homo sapiens)protein:increased expression:peritoneal fluid (human)RGD 

Objects Annotated

Genes (Mus musculus)
Cxcl15  (C-X-C motif chemokine ligand 15)

Genes (Homo sapiens)
CXCL8  (C-X-C motif chemokine ligand 8)


Additional Information