RGD Reference Report - The N-terminus of retinol dehydrogenase type 1 signals cytosolic orientation in the microsomal membrane. - Rat Genome Database

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The N-terminus of retinol dehydrogenase type 1 signals cytosolic orientation in the microsomal membrane.

Authors: Wang, J  Bongianni, J K  Napoli, J L 
Citation: Wang J, etal., Biochemistry. 2001 Oct 23;40(42):12533-40.
RGD ID: 14390050
Pubmed: PMID:11601977   (View Abstract at PubMed)

We determined the orientation of the SDR (short-chain dehydrogenase/reductase) rat RoDH1 (retinol dehydrogenase type 1) in the endoplasmic reticulum to provide insight into its function in retinol metabolism, and to resolve whether retinoid-metabolizing SDRs differ from several other SDRs by requiring a C-terminal segment for the membrane orientation. In contrast to several soluble SDRs, the membrane-associated RoDH1 has hydrophobic extensions N- and C-terminal to the SDR core. Confocal microscopy and/or proteinase K protection assays of RoDH1, RoDH1 mutants, and RoDH1-green fluorescent protein fusion proteins showed that the N-terminal segment anchors RoDH1 to the endoplasmic reticulum membrane facing the cytosol. The C-terminal hydrophobic segment increases the relative proportion of RoDH1 associated with the endoplasmic reticulum, but has no affect on orientation. Deletion of either or both extensions causes nearly total loss of enzyme activity, possibly through altering the nature of RoDH1 association with membranes, or destabilizing the enzyme, but does not alter the expression of RoDH1 or convert it into a soluble protein. The latter suggests that the SDR core of RoDH1 has marked external hydrophobicity that causes nonspecific membrane association.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rdh16Ratendoplasmic reticulum membrane located_inIDA PMID:11601977UniProt 
Rdh16Ratorganelle membrane located_inIDA PMID:11601977UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Rdh16  (retinol dehydrogenase 16)


Additional Information