RGD Reference Report - Identification of a new membrane-bound heparan sulphate proteoglycan. - Rat Genome Database

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Identification of a new membrane-bound heparan sulphate proteoglycan.

Authors: Filmus, J  Shi, W  Wong, Z M  Wong, M J 
Citation: Filmus J, etal., Biochem J. 1995 Oct 15;311 ( Pt 2):561-5.
RGD ID: 13702227
Pubmed: PMID:7487896   (View Abstract at PubMed)
PMCID: PMC1136036   (View Article at PubMed Central)

The morphological changes that occur during intestinal development have been extensively described, but the molecular basis of these changes is largely unknown. As a result of our efforts to identify molecules that play a role in intestinal morphogenesis during development, we have previously isolated a cDNA that is developmentally regulated in the intestine. This cDNA, named OCI-5, was recently shown to have 20-25% identity at the protein-sequence level with glypican and cerebroglycan, two heparan sulphate proteoglycans (HSPG) that are attached to the cell membrane by a glycosyl-phosphatidylinositol (GPI) anchor. Here we provide experimental evidence indicating that OCI-5 is also a GPI-linked HSPG. We demonstrate this by showing that OCI-5 can be labelled with radioactive sulphate and can be digested by heparitinase, but not by chondroitinase. We also show that treatment with phosphatidylinositol-specific phospholipase C releases OCI-5 from the cell surface of COS cells transfected with an OCI-5 expression vector. The identification of OCI-5 as a GPI-linked HSPG confirms that this proteoglycan belongs to the same family of HSPGs that include glypican and cerebroglycan.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gpc3Ratplasma membrane located_inIDA PMID:7487896UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Gpc3  (glypican 3)


Additional Information