RGD Reference Report - Glycine transporters are differentially expressed among CNS cells. - Rat Genome Database

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Glycine transporters are differentially expressed among CNS cells.

Authors: Zafra, F  Aragón, C  Olivares, L  Danbolt, N C  Giménez, C  Storm-Mathisen, J 
Citation: Zafra F, etal., J Neurosci. 1995 May;15(5 Pt 2):3952-69.
RGD ID: 13702203
Pubmed: PMID:7751957   (View Abstract at PubMed)
PMCID: PMC6578198   (View Article at PubMed Central)

Glycine is the major inhibitory neurotransmitter in the spinal cord and brainstem and is also required for the activation of NMDA receptors. The extracellular concentration of this neuroactive amino acid is regulated by at least two glycine transporters (GLYT1 and GLYT2). To study the localization and properties of these proteins, sequence-specific antibodies against the cloned glycine transporters have been raised. Immunoblots show that the 50-70 kDa band corresponding to GLYT1 is expressed at the highest concentrations in the spinal cord, brainstem, diencephalon, and retina, and, in a lesser degree, to the olfactory bulb and brain hemispheres, whereas it is not detected in peripheral tissues. Pre-embedding light and electron microscopic immunocytochemistry show that GLYT1 is expressed in glial cells around both glycinergic and nonglycinergic neurons except in the retina, where it is expressed by amacrine neurons, but not by glia. The expression of a 90-110 kDa band corresponding to GLYT2 is restricted to the spinal cord, brain-stem, and cerebellum; in addition, very low levels occur in the diencephalon. GLYT2 is found in presynaptic elements of neurons thought to be glycinergic. However, in the cerebellum, GLYT2 is expressed both in terminal boutons and in glial elements. The physiological consequences of the regional and cellular distributions of these two proteins as well as the possibility of the existence of an unidentified neuronal form of GLYT1 are discussed.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc6a5Ratglycinergic synapse is_active_inIDA PMID:7751957SynGO 
Slc6a5Ratpresynaptic membrane is_active_inIDA PMID:7751957SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Slc6a5  (solute carrier family 6 member 5)


Additional Information