RGD Reference Report - P2X7 receptors in neurohypophysial terminals: evidence for their role in arginine-vasopressin secretion. - Rat Genome Database

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P2X7 receptors in neurohypophysial terminals: evidence for their role in arginine-vasopressin secretion.

Authors: Cuadra, Adolfo E  Custer, Edward E  Bosworth, Elizabeth L  Lemos, José R 
Citation: Cuadra AE, etal., J Cell Physiol. 2014 Mar;229(3):333-42. doi: 10.1002/jcp.24453.
RGD ID: 13702177
Pubmed: PMID:24037803   (View Abstract at PubMed)
PMCID: PMC4667357   (View Article at PubMed Central)
DOI: DOI:10.1002/jcp.24453   (Journal Full-text)

Arginine-vasopressin (AVP) plays a major role in maintaining cardiovascular function and related pathologies. The mechanism involved in its release into the circulation is complex and highly regulated. Recent work has implicated the purinergic receptor, P2X7R, in a role for catecholamine-enhanced AVP release in the rat hypothalamic-neurohypophysial (NH) system. However, the site of P2X7R action in this endocrine system, and whether or not it directly mediates release in secretory neurons have not been determined. We hypothesized that the P2X7R is expressed and mediates AVP release in NH terminals. P2X7R function was first examined by patch-clamp recordings in isolated NH terminals. Results revealed that subpopulations of isolated terminals displayed either high ATP-sensitivity or low ATP-sensitivity, the latter of which was characteristic of the rat P2X7R. Additional recordings showed that terminals showing sensitivity to the P2X7R-selective agonist, BzATP, were further inhibited by P2X7R selective antagonists, AZ10606120 and brilliant blue-G. In confocal micrographs from tissue sections and isolated terminals of the NH P2X7R-immunoreactivity was found to be localized in plasma membranes. Lastly, the role of P2X7R on AVP release was tested. Our results showed that BzATP evoked sustained AVP release in NH terminals, which was inhibited by AZ10606120. Taken together, our data lead us to conclude that the P2X7R is expressed in NH terminals and corroborates its role in AVP secretion.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
P2rx7Ratregulation of presynaptic dense core granule exocytosis involved_inEXP PMID:24037803SynGO 
P2rx7Ratregulation of presynaptic dense core granule exocytosis involved_inIDA PMID:24037803SynGO 

Objects Annotated

Genes (Rattus norvegicus)
P2rx7  (purinergic receptor P2X 7)


Additional Information