RGD Reference Report - Transmembrane agrin regulates dendritic filopodia and synapse formation in mature hippocampal neuron cultures. - Rat Genome Database

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Transmembrane agrin regulates dendritic filopodia and synapse formation in mature hippocampal neuron cultures.

Authors: McCroskery, S  Bailey, A  Lin, L  Daniels, M P 
Citation: McCroskery S, etal., Neuroscience. 2009 Sep 29;163(1):168-79. doi: 10.1016/j.neuroscience.2009.06.012. Epub 2009 Jun 10.
RGD ID: 13702171
Pubmed: PMID:19524020   (View Abstract at PubMed)
PMCID: PMC2728160   (View Article at PubMed Central)
DOI: DOI:10.1016/j.neuroscience.2009.06.012   (Journal Full-text)

The transmembrane isoform of agrin (Tm-agrin) is the predominant form expressed in the brain but its putative roles in brain development are not well understood. Recent reports have implicated Tm-agrin in the formation and stabilization of filopodia on neurites of immature central and peripheral neurons in culture. In maturing central neurons, dendritic filopodia are believed to facilitate synapse formation. In the present study we have investigated the role of Tm-agrin in regulation of dendritic filopodia and synaptogenesis in maturing cultures of rat hippocampal neurons. We did this by infecting the neurons with an RNAi lentivirus to deplete endogenous agrin during the developmental period when filopodia density on the dendritic arbor was high, and synapse formation was rapid. We found that dendritic filopodia density was markedly reduced, as was synapse density along dendrites. Moreover, synapse formation was more sharply reduced on dendrites of infected neurons contacted by uninfected axons than on uninfected dendrites contacted by infected axons. The results are consistent with a physiological role for Tm-agrin in the maturation of hippocampal neurons involving positive regulation of dendritic filopodia and consequent promotion of synaptogenesis, but also suggest a role for axonal agrin in synaptogenesis.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AgrnRatregulation of synapse organization involved_inIDA PMID:19524020SynGO 
AgrnRatregulation of synapse organization involved_inIMP PMID:19524020SynGO 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AgrnRatglutamatergic synapse is_active_inIDA PMID:19524020SynGO 
AgrnRatglutamatergic synapse is_active_inIMP PMID:19524020SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Agrn  (agrin)


Additional Information