RGD Reference Report - Mammary inflammation around parturition appeared to be attenuated by consumption of fish oil rich in n-3 polyunsaturated fatty acids. - Rat Genome Database

RGD Reference Report - Mammary inflammation around parturition appeared to be attenuated by consumption of fish oil rich in n-3 polyunsaturated fatty acids. - Rat Genome Database

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Mammary inflammation around parturition appeared to be attenuated by consumption of fish oil rich in n-3 polyunsaturated fatty acids.
Authors:	Lin, Sen Hou, Jia Xiang, Fang Zhang, Xiaoling Che, Lianqiang Lin, Yan Xu, Shengyu Tian, Gang Zeng, Qiufeng Yu, Bing Zhang, Keying Chen, Daiwen Wu, De Fang, Zhengfeng
Citation:	Lin S, etal., Lipids Health Dis. 2013 Dec 31;12:190. doi: 10.1186/1476-511X-12-190.
RGD ID:	13210508
Pubmed:	PMID:24378112 (View Abstract at PubMed)
PMCID:	PMC3896666 (View Article at PubMed Central)
DOI:	DOI:10.1186/1476-511X-12-190 (Journal Full-text)
BACKGROUND: Mastitis endangers the health of domestic animals and humans, and may cause problems concerning food safety. It is documented that n-3 polyunsaturated fatty acids (PUFA) play significant roles in attenuating saturated fatty acids (SFA)-induced inflammation. This study was therefore conducted to determine whether mammary inflammation could be affected by consumption of diets rich in n-3 PUFA. METHODS: Forty-eight rats after mating began to receive diets supplemented with 5% fish oil (FO) or 7% soybean oil (SO). Blood and mammary tissue samples (n¿=¿6) at day 0 and 14 of gestation and day 3 postpartum were collected 9 hours after intramammary infusion of saline or lipopolysaccharide (LPS) to determine free fatty acids (FFA) concentration and FA composition in plasma and inflammation mediators in mammary tissues. RESULTS: At day 14 of gestation and day 3 postpartum, the FO-fed rats had lower plasma concentrations of C18:2n6, C20:4n6, total n-6 PUFA and SFA, and higher plasma concentrations of C20:5n3 and total n-3 PUFA than the SO-fed rats. Plasma C22:6n3 concentration was also higher in the FO-fed than in the SO-fed rats at day 3 postpartum. Compared with the SO-fed rats, the FO-fed rats had lower mammary mRNA abundance of xanthine oxidoreductase (XOR) and protein level of tumor necrosis factor (TNF)-α, but had higher mammary mRNA abundances of interleukin (IL)-10 and peroxisome proliferator-activated receptor (PPAR)-γ at day 14 of gestation. Following LPS infusion at day 3 postpartum, the SO-fed rats had increased plasma concentrations of FFA, C18:1n9, C18:3n3, C18:2n6 and total n-6 PUFA, higher mammary mRNA abundances of IL-1ß, TNF-α and XOR but lower mammary mRNA abundance of IL-10 than the FO-fed rats. CONCLUSIONS: Mammary inflammation around parturition appeared to be attenuated by consumption of a diet rich in n-3 PUFA, which was associated with up-regulated expression of IL-10 and PPAR-γ.

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
Xdh	Rat	response to lipopolysaccharide		IEP			RGD

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Genes (Rattus norvegicus)

Xdh (xanthine dehydrogenase)

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