RGD Reference Report - The adenosine A2A receptor interacts with the actin-binding protein alpha-actinin. - Rat Genome Database

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The adenosine A2A receptor interacts with the actin-binding protein alpha-actinin.

Authors: Burgueno, J  Blake, DJ  Benson, MA  Tinsley, CL  Esapa, CT  Canela, EI  Penela, P  Mallol, J  Mayor F, JR  Lluis, C  Franco, R  Ciruela, F 
Citation: Burgueno J, etal., J Biol Chem 2003 Sep 26;278(39):37545-52. Epub 2003 Jul 01.
RGD ID: 1304399
Pubmed: PMID:12837758   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M302809200   (Journal Full-text)

Recently, evidence has emerged that heptaspanning membrane or G protein-coupled receptors may be linked to intracellular proteins identified as regulators of receptor anchoring and signaling. Using a yeast two-hybrid screen, we identified alpha-actinin, a major F-actin-cross-linking protein, as a binding partner for the C-terminal domain of the adenosine A2A receptor (A2AR). Colocalization, co-immunoprecipitation, and pull-down experiments showed a close and specific interaction between A2AR and alpha-actinin in transfected HEK-293 cells and also in rat striatal tissue. A2AR activation by agonist induced the internalization of the receptor by a process that involved rapid beta-arrestin translocation from the cytoplasm to the cell surface. In the subsequent receptor traffic from the cell surface, the role of actin organization was shown to be crucial in transiently transfected HEK-293 cells, as actin depolymerization by cytochalasin D prevented its agonist-induced internalization. A2ADeltaCTR, a mutant version of A2AR that lacks the C-terminal domain and does not interact with alpha-actinin, was not able to internalize when activated by agonist. Interestingly, A2ADeltaCTR did not show aggregation or clustering after agonist stimulation, a process readily occurring with the wild-type receptor. These findings suggest an alpha-actinin-dependent association between the actin cytoskeleton and A2AR trafficking.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
alpha-actinin binding  IDA 1304399in primary rat striatal neuronsRGD 

Objects Annotated

Genes (Rattus norvegicus)
Adora2a  (adenosine A2a receptor)


Additional Information