RGD Reference Report - Characterization of two novel proteins, NgRH1 and NgRH2, structurally and biochemically homologous to the Nogo-66 receptor. - Rat Genome Database

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Characterization of two novel proteins, NgRH1 and NgRH2, structurally and biochemically homologous to the Nogo-66 receptor.

Authors: Pignot, V  Hein, AE  Barske, C  Wiessner, C  Walmsley, AR  Kaupmann, K  Mayeur, H  Sommer, B  Mir, AK  Frentzel, S 
Citation: Pignot V, etal., J Neurochem 2003 May;85(3):717-28.
RGD ID: 1299553
Pubmed: PMID:12694398   (View Abstract at PubMed)

Nogo-66 receptor (NgR) has recently been identified as the neuronal receptor of the myelin-associated proteins Nogo-A, oligodendrocyte protein (OMgp) and myelin-associated glycoprotein (MAG), and mediates inhibition of axonal regeneration both in vitro and in vivo. Through database searches, we have identified two novel proteins (NgRH1 and NgRH2) that turned out to be homologous in their primary structures, biochemical properties and expression patterns to NgR. Like NgR, the homologues contain eight leucine-rich repeats (LRR) flanked by a leucine-rich repeat C-terminus (LRRCT) and a leucine-rich repeat N-terminus (LRRNT), and also have a C-terminal GPI signal sequence. Northern blot analysis showed predominant expression of NgRH1 and NgRH2 mRNA in the brain. In situ hybridization and immunohistochemistry on rat brain slices revealed neuronal expression of the genes. NgRH1 and NgRH2 were detected on the cell surface of recombinant cell lines as N-glycosylated GPI anchored proteins and, consistent with other GPI anchored proteins, were localized within the lipid rafts of cellular membranes. In addition, an N-terminal proteolytic fragment of NgR comprising the majority of the ectodomain was found to be constitutively secreted from cells. Our data indicate that NgR, NgRH1 and NgRH2 constitute a novel receptor protein family, which may play related roles within the CNS.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rtn4rl2Ratcell surface receptor signaling pathway  NAS  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rtn4rl2Ratexternal side of plasma membrane  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rtn4rl2  (reticulon 4 receptor-like 2)

Objects referenced in this article
Gene Rtn4rl1 reticulon 4 receptor-like 1 Mus musculus
Gene Rtn4rl2 reticulon 4 receptor-like 2 Mus musculus
Gene Rtn4rl1 reticulon 4 receptor-like 1 Rattus norvegicus

Additional Information