RGD Reference Report - Time-dependent changes in expression of troponin subunit isoforms in unloaded rat soleus muscle. - Rat Genome Database

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Time-dependent changes in expression of troponin subunit isoforms in unloaded rat soleus muscle.

Authors: Stevens, L  Bastide, B  Kischel, P  Pette, D  Mounier, Y 
Citation: Stevens L, etal., Am J Physiol Cell Physiol 2002 May;282(5):C1025-30.
RGD ID: 1299083
Pubmed: PMID:11940518   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpcell.00252.2001   (Journal Full-text)

This study focuses on the effects of mechanical unloading of rat soleus muscle on the isoform patterns of the three troponin (Tn) subunits: troponin T (TnT), troponin I (TnI), and troponin C (TnC). Mechanical unloading was achieved by hindlimb unloading (HU) for time periods of 7, 15, and 28 days. Relative concentrations of slow and fast TnT, TnI, and TnC isoforms were assessed by electrophoretic and immunoblot analyses. HU induced profound slow-to-fast isoform transitions of all Tn subunits, although to different extents and with different time courses. The effectiveness of the isoform transitions was higher for TnT than for TnI and TnC. Indeed, TnI and TnC encompassed minor partial exchanges of slow isoforms with their fast counterparts, whereas the expression pattern of fast TnT isoforms (TnTf) was largely increased after HU. Moreover, slow and fast isoforms of the different Tn were not affected in the same manner by HU. This suggests that the slow and fast counterparts of the Tn subunit isoforms are regulated independently in response to HU. The changes in TnTf composition occurred in parallel with previously demonstrated transitions within the pattern of the fast myosin heavy chains in the same muscles.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Tnnt3Ratmuscle contraction  TAS  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Tnnt3Rattroponin complex  TAS  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Tnnt3  (troponin T3, fast skeletal type)


Additional Information