RGD Reference Report - NBD peptides protect against ischemia reperfusion after orthotopic liver transplantation in rats. - Rat Genome Database

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NBD peptides protect against ischemia reperfusion after orthotopic liver transplantation in rats.

Authors: Cheng, Ming-Xiang  Gong, Jian-Ping  Chen, Yong  Liu, Zuo-Jin  Tu, Bing  Liu, Chang-An 
Citation: Cheng MX, etal., J Surg Res. 2012 Aug;176(2):666-71. doi: 10.1016/j.jss.2011.12.005. Epub 2011 Dec 22.
RGD ID: 12791280
Pubmed: PMID:22381173   (View Abstract at PubMed)
DOI: DOI:10.1016/j.jss.2011.12.005   (Journal Full-text)


BACKGROUND: NBD (NEMO binding domain) peptides could selectively inhibit the inflammation induced NF-κB activity, while sparing the protective functions of basal NF-κB activity. The aim of this study was to determine whether NBD peptides inhibited the transcriptional activity of nuclear factor-κB (NF-κB) during liver transplant ischemia-reperfusion injury (IRI), without affecting its basal function.
MATERIALS AND METHODS: Sprague Dawley (SD) rats were performed orthotropic liver transplantation according to the Kamada technique. Donors were given NBD peptides (8 mg/kg, intraperitoneal) 2 h before surgery (n = 24) and the controls were treated with the same volume of physiologic saline (n = 24). An additional 16 animals in normal condition (did not undergo any surgery) were also divided into two groups and given the same treatment as above to assess the effect of NBD peptides on basal function. We analyzed levels of alanine aminotransferase (ALT), tumor necrosis factor (TNF-α), IKK (IκB kinase) complex phosphorylation, IκBα degradation, NF-κB transcriptional activity, apoptosis, and performed a morphologic study of liver tissues at 3, 6, and 24 h after portal vein reperfusion and in normal condition (n = 8).
RESULTS: Pretreatment with NBD peptides significantly improved liver function, attenuating liver parenchymal cell damage, apoptosis by down-regulating TNF-α level, inhibiting IKK complex phosphorylation, IκBα degradation, and NF-κB transcriptional activity, but had no effect in normal condition.
CONCLUSION: NBD peptides attenuated hepatic IRI by preventing NF-κB activation, without affecting basal NF-κB activity.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IKBKGHumanLiver Reperfusion Injury treatmentISOIkbkg (Rattus norvegicus) RGD 
IkbkgRatLiver Reperfusion Injury treatmentIDA  RGD 
IkbkgMouseLiver Reperfusion Injury treatmentISOIkbkg (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ikbkg  (inhibitor of nuclear factor kappa B kinase regulatory subunit gamma)

Genes (Mus musculus)
Ikbkg  (inhibitor of kappaB kinase gamma)

Genes (Homo sapiens)
IKBKG  (inhibitor of nuclear factor kappa B kinase regulatory subunit gamma)


Additional Information