RGD Reference Report - Postnatal regulation of 15-hydroxyprostaglandin dehydrogenase in the rat kidney. - Rat Genome Database

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Postnatal regulation of 15-hydroxyprostaglandin dehydrogenase in the rat kidney.

Authors: Liu, Ying  Jia, Zhanjun  Sun, Ying  Zhou, Li  Downton, Maicy  Chen, Ren  Zhang, Aihua  Yang, Tianxin 
Citation: Liu Y, etal., Am J Physiol Renal Physiol. 2014 Aug 15;307(4):F388-95. doi: 10.1152/ajprenal.00512.2013. Epub 2014 Mar 19.
RGD ID: 11667087
Pubmed: PMID:24647712   (View Abstract at PubMed)
PMCID: PMC4137130   (View Article at PubMed Central)
DOI: DOI:10.1152/ajprenal.00512.2013   (Journal Full-text)

Cyclooxygenase 2 (COX-2) has an established role in postnatal kidney development. 15-Hydroxyprostaglandin dehydrogenase (15-PGDH) is recently identified as an endogenous inhibitor of COX-2, limiting the production of COX-2-derived prostanoids in several pathological conditions. The present study was undertaken to examine the regulation of renal 15-PGDH expression during postnatal kidney development in rats compared with COX-2. qRT-PCR and immunoblotting demonstrated that 15-PGDH mRNA and protein in the kidney were present in neonates, peaked in the second postnatal week, and then declined sharply to very low level in adulthood. Immunostaining demonstrated that at the second postnatal week, renal 15-PGDH protein was predominantly found in the proximal tubules stained positive for Na/H exchanger 3 and brush borders (periodic acid-Schiff), whereas COX-2 protein was restricted to macular densa and adjacent thick ascending limbs. Interestingly, in the fourth postnatal week, 15-PGDH protein was redistributed to thick ascending limbs stained positive for the Na-K-2Cl cotransporter. After 6 wk of age, 15-PGDH protein was found in the granules in subsets of the proximal tubules. Overall, these results support a possibility that 15-PGDH may regulate postnatal kidney development through interaction with COX-2.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
kidney development  IEP 11667087 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
extracellular space  IDA 11667087 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hpgd  (15-hydroxyprostaglandin dehydrogenase)


Additional Information