RGD Reference Report - Calcium signalling in the regulation of PGC-1alpha, PDK4 and HKII mRNA expression. - Rat Genome Database

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Calcium signalling in the regulation of PGC-1alpha, PDK4 and HKII mRNA expression.

Authors: Kusuhara, K  Madsen, K  Jensen, L  Hellsten, Y  Pilegaard, H 
Citation: Kusuhara K, etal., Biol Chem. 2007 May;388(5):481-8.
RGD ID: 10059651
Pubmed: PMID:17516843   (View Abstract at PubMed)
DOI: DOI:10.1515/BC.2007.052   (Journal Full-text)

The role of calcium signalling and specific intracellular calcium signalling pathways in regulating skeletal muscle tissue peroxisome proliferator-activated receptor gamma co-activator (PGC)-1alpha, hexokinase (HK)II and pyruvate dehydrogenase kinase (PDK)4 mRNA was examined. Cultured primary rat skeletal muscle cells were incubated for 6 h in caffeine or ionomycin. Because PGC-1alpha mRNA clearly showed greater induction with ionomycin, the latter was chosen for the main experiments, whereby cells were incubated for 6 h with either ionomycin alone or in combination with either cyclosporin A or KN-62. The PGC-1alpha mRNA level was increased (p<0.05) approximately six-fold and HKII mRNA content approximately two-fold by ionomycin relative to the corresponding controls, whereas the PDK4 mRNA content remained unaffected. Cyclosporin A abolished (p<0.05) and KN-62 reduced (p<0.1) the ionomycin-induced increase in PGC-1alpha mRNA. Electrical stimulation of in vitro incubated rat EDL muscle increased (p<0.05) PGC-1alpha mRNA by 2.2-fold after 4 h of recovery relative to a resting control, and this increase was absent when muscles were incubated with KN-62 or cyclosporin A. The present data strongly suggest that calcium signalling is involved in regulating the PGC-1alpha and HKII genes, but not PDK4. Both calcineurin and CaMK signalling seem to be involved in the calcium- and contraction-mediated PGC-1alpha up-regulation in skeletal muscle.




Biological Process

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Ppargc1aRatcellular response to caffeine  IEP  RGD 
Ppargc1aRatcellular response to ionomycin  IEP  RGD 


Genes (Rattus norvegicus)
Ppargc1a  (PPARG coactivator 1 alpha)