RGD Reference Report - Hypoxia and cytoplasmic alkalinization upregulate growth hormone expression in lymphocytes. - Rat Genome Database

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Hypoxia and cytoplasmic alkalinization upregulate growth hormone expression in lymphocytes.

Authors: Weigent, DA 
Citation: Weigent DA Cell Immunol. 2013 Mar;282(1):9-16. doi: 10.1016/j.cellimm.2013.03.007. Epub 2013 Apr 11.
RGD ID: 10003149
Pubmed: PMID:23639351   (View Abstract at PubMed)
PMCID: PMC3693391   (View Article at PubMed Central)
DOI: DOI:10.1016/j.cellimm.2013.03.007   (Journal Full-text)

We report here that culture of lymphoid cells under hypoxic conditions showed an increase in both luciferase expression from a GH-promoter luciferase construct and the levels of lymphocyte GH. The effect was mimicked by treatment of cells with cobalt chloride consistent with a specific oxygen-sensing mechanism. We identified a putative hypoxia response element (HRE) in the GH promoter at the region -176 bp to -172 bp that contains a copy of the hypoxia-inducible factor-1 (Hif-1) binding motif (5'-ACGTG-3'). The results also showed that culture of primary rat spleen cells with different doses of TMA induced a dose-dependent increase in lymphocyte GH by Western blot analysis. Greater levels of GH are induced in T cell-enriched populations compared to B cell-enriched populations after treatment with CoCl(2) or TMA. Our results suggest that the stressful cellular conditions likely to occur at sites of inflammation or tumor growth may induce the synthesis of lymphocyte GH.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gh1Ratcellular response to alkaline pH  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gh1  (growth hormone 1)


Additional Information