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MEASUREMENT METHODS - ANNOTATIONS

The Clinical Measurement Ontology (CMO), Measurement Methods Ontology (MMO), and Experimental Condition Ontology (XCO) are currently being developed at the Rat Genome Database. For more information about these vocabularies please see Shimoyama et al. Three ontologies to define phenotype measurement data. Front Genet. 2012;3:87. Epub 2012 May 28 or contact us (http://rgd.mcw.edu/contact/index.shtml).
Term:nitrite analysis using the Griess reaction
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Accession:MMO:0000370 term browser browse the term
Definition:Method of assessing the amount of nitrite, the nitrogen oxoanion chemical formula NO2(-), in a solution by treating with a diazotizing reagent, e.g. sulfanilamide (SA), in acidic media to form a transient diazonium salt which is then allowed to react with a coupling reagent, N-naphthyl-ethylenediamine (NED), to form a stable azo compound with an intense purple color that absorbs light at 540 nm. Measurement of nitrite is used to estimate nitric oxide (NO) production in biological systems.
Synonyms:related_synonym: Griess test;   Griess test for nitric oxide;   nitric oxide analysis using the Griess reaction;   nitrite analysis using the Griess reagent


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  measurement method 2368
    ex vivo method 1236
      spectrometry 56
        photometry 40
          colorimetry 40
            liquid phase colorimetry 36
              nitrite analysis by liquid phase colorimetry 0
                nitrite analysis using the Griess reaction 0
                  blood nitrite analysis using the Griess reaction 0
                  exudate nitrite analysis using the Griess reaction 0
                  urine nitrite analysis using the Griess reaction 0
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