Catalysis of the reaction: a short-chain 2,3-saturated fatty acyl-CoA + O2 = a short-chain (2E)-enoyl-CoA + H2O2.
Comment:
While there is not universal consensus on the lengths of short-, medium-, long- and very-long-chain fatty acids, the GO uses the definitions in ChEBI (see CHEBI:26666, CHEBI:59554, CHEBI:15904 and CHEBI:27283).