RGD Reference Report - Up-regulation of IL-18 and IL-12 in the ileum of neonatal rats with necrotizing enterocolitis. - Rat Genome Database

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Up-regulation of IL-18 and IL-12 in the ileum of neonatal rats with necrotizing enterocolitis.

Authors: Halpern, MD  Holubec, H  Dominguez, JA  Williams, CS  Meza, YG  McWilliam, DL  Payne, CM  McCuskey, RS  Besselsen, DG  Dvorak, B 
Citation: Halpern MD, etal., Pediatr Res 2002 Jun;51(6):733-9.
RGD ID: 729222
Pubmed: PMID:12032269   (View Abstract at PubMed)
DOI: DOI:10.1203/00006450-200206000-00012   (Journal Full-text)

Necrotizing enterocolitis (NEC) is a common and devastating gastrointestinal disease of premature infants. Because the proinflammatory cytokines IL-18, IL-12, and interferon (IFN)-gamma have been implicated in other diseases of the small intestine, we hypothesized that these cytokines would play an important role in NEC pathogenesis. NEC was induced in newborn rats via enteral feeding with rat milk substitute and asphyxia and cold stress (RMS). Dam-fed, asphyxia- and cold-stressed littermates were used as controls (DF). After 96 h, the distal ileum was removed from all animals and processed to determine expression and localization of IL-18, IL-12, and IFN-gamma using real-time reverse transcriptase PCR and immunohistology. IL-18 and IL-12 mRNA from the RMS group were increased (p < or = 0.05) compared with DF controls, and there was a correlation between increasing IL-18 and IL-12 mRNA levels and progression of tissue damage (r = 0.629 and 0.588, respectively; p < or = 0.05). Immunohistology revealed IL-18 in the cytoplasm of villi and crypt enterocytes and IL-12-positive monocytes/macrophages were increased with disease progression (r = 0.503, p < or = 0.05). No differences in the number of IFN-gamma-positive cells were observed between groups. These data demonstrate up-regulation of IL-18 and IL-12 in experimental NEC and a correlation between production of these proinflammatory cytokines and progression of tissue damage.



RGD Manual Disease Annotations    Click to see Annotation Detail View
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IL18Humanperinatal necrotizing enterocolitis  ISOIl18 (Rattus norvegicus)protein:increased expression:ileumRGD 
Il18Ratperinatal necrotizing enterocolitis  IEP protein:increased expression:ileumRGD 
Il18Mouseperinatal necrotizing enterocolitis  ISOIl18 (Rattus norvegicus)protein:increased expression:ileumRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Il18Ratapical plasma membrane  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Il18  (interleukin 18)

Genes (Mus musculus)
Il18  (interleukin 18)

Genes (Homo sapiens)
IL18  (interleukin 18)

Objects referenced in this article
Gene Il12b interleukin 12B Rattus norvegicus

Additional Information