RGD Reference Report - Identification of a novel RING finger protein as a coregulator in steroid receptor-mediated gene transcription. - Rat Genome Database

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Identification of a novel RING finger protein as a coregulator in steroid receptor-mediated gene transcription.

Authors: Moilanen, AM  Poukka, H  Karvonen, U  Hakli, M  Janne, OA  Palvimo, JJ 
Citation: Moilanen AM, etal., Mol Cell Biol 1998 Sep;18(9):5128-39.
RGD ID: 69969
Pubmed: PMID:9710597   (View Abstract at PubMed)
PMCID: PMC109098   (View Article at PubMed Central)

Using the DNA-binding domain of androgen receptor (AR) as a bait in a yeast two-hybrid screening, we have identified a small nuclear RING finger protein, termed SNURF, that interacts with AR in a hormone-dependent fashion in both yeast and mammalian cells. Physical interaction between AR and SNURF was demonstrated by coimmunoprecipitation from cell extracts and by protein-protein affinity chromatography. Rat SNURF is a highly hydrophilic protein consisting of 194 amino acid residues and comprising a consensus C3HC4 zinc finger (RING) structure in the C-terminal region and a bipartite nuclear localization signal near the N terminus. Immunohistochemical experiments indicated that SNURF is a nuclear protein. SNURF mRNA is expressed in a variety of human and rat tissues. Overexpression of SNURF in cultured mammalian cells enhanced not only androgen, glucocorticoid, and progesterone receptor-dependent transactivation but also basal transcription from steroid-regulated promoters. Mutation of two of the potential Zn2+ coordinating cysteines to serines in the RING finger completely abolished the ability of SNURF to enhance basal transcription, whereas its ability to activate steroid receptor-dependent transcription was maintained, suggesting that there are separate domains in SNURF that mediate interactions with different regulatory factors. SNURF is capable of interacting in vitro with the TATA-binding protein, and the RING finger domain is needed for this interaction. Collectively, we have identified and characterized a ubiquitously expressed RING finger protein, SNURF, that may function as a bridging factor and regulate steroid receptor-dependent transcription by a mechanism different from those of previously identified coactivator or integrator proteins.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to testosterone stimulus  IDA 69969 RGD 
positive regulation of transcription by RNA polymerase II  IDA 69969 RGD 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Ar  (androgen receptor)
Rnf4  (ring finger protein 4)

Objects referenced in this article
Gene Rnf4 ring finger protein 4 Mus musculus

Additional Information