RGD Reference Report - A new mouse model of lung allergy induced by the spores of Alternaria alternata and Cladosporium herbarum molds. - Rat Genome Database

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A new mouse model of lung allergy induced by the spores of Alternaria alternata and Cladosporium herbarum molds.

Authors: Havaux, X  Zeine, A  Dits, A  Denis, O 
Citation: Havaux X, etal., Clin Exp Immunol. 2005 Feb;139(2):179-88.
RGD ID: 5688158
Pubmed: PMID:15654816   (View Abstract at PubMed)
PMCID: PMC1809297   (View Article at PubMed Central)
DOI: DOI:10.1111/j.1365-2249.2004.02679.x   (Journal Full-text)

Asthma is a serious health problem and during the last decade various experimental models of asthma have been developed to study the pathogenesis of this disease. In this study we describe a new mouse model of asthma that uses the spores of Alternaria alternata and Cladosporium herbarum, two allergenic molds recognized as common inducers of rhinitis and asthma in humans. Here we demonstrate that A. alternata and C. herbarum spores are immunogenic when injected into BALB/c mice, and induce the production of specific IgM and IgG1 antibodies and strongly increase IgE serum levels. To induce the allergic response, mice were sensitized by two intraperitoneal (i.p.) injections and then intranasaly (i.n.) challenged with A. alternata and C. herbarum spores. Bronchoalveolar lavages (BALs) from these mice contained numerous macrophages, neutrophils, eosinophils and lymphocytes whereas neutrophils were the predominant BAL inflammatory cells in nonsensitized mice. Histological studies demonstrated an influx of eosinophils in peri-vascular and peri-bronchial areas and the presence of numerous epithelial goblet cells only in sensitized mice. Increased expression of mRNA specific for various chemokines (eotaxin, MIP-1alpha, MIP-2) and chemokine receptors (CCR-1, CCR-2 and CCR-5) was observed in the lungs of nonsensitized mice challenged with the spores. Expression of CCR-3 mRNA in the lungs and Th2 cytokine (IL-4, IL-5 and IL-13) secretion in the BAL was additionally observed in sensitized and challenged mice. Finally we demonstrate through whole-body plethysmography that mold spore sensitization and challenge induce the development of an airway hyperreactivity in response to nebulized methacholine.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
asthma  ISOCcr1 (Mus musculus)5688158; 5688158mRNA:increased expression:lungRGD 
asthma  IEP 5688158mRNA:increased expression:lungRGD 

Objects Annotated

Genes (Rattus norvegicus)
Ccr1  (C-C motif chemokine receptor 1)

Genes (Mus musculus)
Ccr1  (C-C motif chemokine receptor 1)

Genes (Homo sapiens)
CCR1  (C-C motif chemokine receptor 1)


Additional Information