RGD Reference Report - Subcellular localization of patched and smoothened, the receptors for sonic hedgehog signaling, in the hippocampal neuron. - Rat Genome Database

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Subcellular localization of patched and smoothened, the receptors for sonic hedgehog signaling, in the hippocampal neuron.

Authors: Petralia, RS  Schwartz, CM  Wang, YX  Mattson, MP  Yao, PJ 
Citation: Petralia RS, etal., J Comp Neurol. 2011 Dec 15;519(18):3684-99. doi: 10.1002/cne.22681.
RGD ID: 5509937
Pubmed: PMID:21618238   (View Abstract at PubMed)
PMCID: PMC3196849   (View Article at PubMed Central)
DOI: DOI:10.1002/cne.22681   (Journal Full-text)

Cumulative evidence suggests that, aside from patterning the embryonic neural tube, Sonic hedgehog (Shh) signaling plays important roles in the mature nervous system. In this study, we investigate the expression and localization of the Shh signaling receptors, Patched (Ptch) and Smoothened (Smo), in the hippocampal neurons of young and mature rats. Reverse transcriptase-polymerase chain reaction and immunoblotting analyses show that the expression of Ptch and Smo remains at a moderate level in young postnatal and adult brains. By using immunofluorescence light microscopy and immunoelectron microscopy, we examine the spatial distribution of Ptch and Smo within the hippocampal neurons. In young developing neurons, Ptch and Smo are present in the processes and are clustered at their growth cones. In mature neurons, Ptch and Smo are concentrated in dendrites, spines, and postsynaptic sites. Synaptic Ptch and Smo often co-exist with unusual structures-synaptic spinules and autophagosomes. Our results reveal the anatomical organization of the Shh receptors within both the young and the mature hippocampal neurons. J. Comp. Neurol. 519:3684-3699, 2011. (c)2011 Wiley-Liss, Inc.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
axonal growth cone  IDA 5509937; 5509937young hippocampal neuronsRGD 
dendrite  IDA 5509937mature hippocampal neuronsRGD 
dendritic growth cone  IDA 5509937; 5509937young hippocampal neuronsRGD 
postsynapse is_active_inIDA 5509937PMID:21618238SynGO 
postsynaptic membrane is_active_inIDA 5509937PMID:21618238SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Ptch1  (patched 1)
Smo  (smoothened, frizzled class receptor)


Additional Information