RGD Reference Report - Gene and protein expression associated with protein synthesis and breakdown in paraplegic skeletal muscle. - Rat Genome Database

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Gene and protein expression associated with protein synthesis and breakdown in paraplegic skeletal muscle.

Authors: Drummond, MJ  Glynn, EL  Lujan, HL  DiCarlo, SE  Rasmussen, BB 
Citation: Drummond MJ, etal., Muscle Nerve. 2008 Apr;37(4):505-13.
RGD ID: 5509082
Pubmed: PMID:18236467   (View Abstract at PubMed)
PMCID: PMC2715297   (View Article at PubMed Central)
DOI: DOI:10.1002/mus.20976   (Journal Full-text)

Spinal cord injury reduces the rate of skeletal muscle protein synthesis and increases protein breakdown, resulting in rapid muscle loss. The purpose of this study was to determine whether long-term paraplegia would eventually result in a downregulation of muscle mRNA and protein expression associated with both protein synthesis and breakdown. After 10 weeks of spinal cord transection, soleus muscle from 12 rats (6 sham-control, 6 paraplegic) was studied for mRNAs and proteins associated with protein synthesis and breakdown using real-time polymerase chain reaction and immunoblotting techniques. Protein kinase B (PKB/Akt), ribosomal S6 kinase 1 (S6K1), and myogenin mRNA were downregulated, whereas muscle ring finger 1 (MuRF1) and phospho-forkhead transcription factor 4 (FoxO4) protein were increased in paraplegic rats. We conclude that gene and protein expression of pathways associated with protein synthesis are reduced, whereas some markers of protein breakdown remain elevated following chronic paraplegia. Clinical interventions designed to increase muscle protein synthesis may be helpful in preventing excessive muscle loss during long-term paraplegia.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
paraplegia  ISOAkt1 (Rattus norvegicus)5509082; 5509082mRNA:decreased expression:soleusRGD 
paraplegia  IEP 5509082mRNA:decreased expression:soleusRGD 
paraplegia  ISOFoxo4 (Rattus norvegicus)5509082; 5509082protein:increased expression:skeletal muscle:RGD 
paraplegia  IEP 5509082protein:increased expression:skeletal muscle:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Akt1  (AKT serine/threonine kinase 1)
Foxo4  (forkhead box O4)

Genes (Mus musculus)
Akt1  (thymoma viral proto-oncogene 1)
Foxo4  (forkhead box O4)

Genes (Homo sapiens)
AKT1  (AKT serine/threonine kinase 1)
FOXO4  (forkhead box O4)


Additional Information