RGD Reference Report - Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes. - Rat Genome Database

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Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes.

Authors: Garcia-Diaz, DF  Campion, J  Milagro, FI  Boque, N  Moreno-Aliaga, MJ  Martinez, JA 
Citation: Garcia-Diaz DF, etal., J Mol Endocrinol. 2010 Jul;45(1):33-43. Epub 2010 Apr 16.
RGD ID: 5128594
Pubmed: PMID:20400526   (View Abstract at PubMed)
DOI: DOI:10.1677/JME-09-0160   (Journal Full-text)

Antioxidant-based treatments are emerging as an interesting approach to possibly counteract obesity fat accumulation complications, since this is accompanied by an increased systemic oxidative stress. The aim of this study was to analyze specific metabolic effects of vitamin C (VC) on epididymal primary rat adipocytes. Cells were isolated and incubated for 72 h in culture medium, in the absence or presence of 1.6 nM insulin, within a range of VC concentrations (5-1000 microM). Glucose- and lipid-related variables as well as the secretion/expression patterns of several obesity-related genes were assessed. It was observed that VC dose dependently inhibited glucose uptake and lactate production, and also reduced glycerol release in both control and insulin-treated cells. Also, VC caused a dramatic concentration-dependent fall in leptin secretion especially in insulin-stimulated cells. In addition, VC (200 microM) induced Cdkn1a and Casp8, partially inhibited Irs3, and together with insulin drastically reduced Gpdh (listed as Gpd1 in the MGI database) gene expressions. Finally, VC and insulin down-regulatory effects were observed on extracellular and intracellular reactive oxygen species production respectively. In summary, this experimental assay describes a specific effect of VC in isolated rat adipocytes on glucose and fat metabolism, and on the secretion/expression of important obesity-related proteins.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to L-ascorbic acid  IEP 5128594 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Lep  (leptin)


Additional Information