RGD Reference Report - Cytokine expression after vaginal distention of different durations in virgin Sprague-Dawley rats. - Rat Genome Database

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Cytokine expression after vaginal distention of different durations in virgin Sprague-Dawley rats.

Authors: Wood, HM  Kuang, M  Woo, L  Hijaz, A  Butler, RS  Penn, M  Rackley, R  Damaser, MS 
Citation: Wood HM, etal., J Urol. 2008 Aug;180(2):753-9. Epub 2008 Jun 13.
RGD ID: 4890426
Pubmed: PMID:18554634   (View Abstract at PubMed)
PMCID: PMC2728595   (View Article at PubMed Central)
DOI: DOI:10.1016/j.juro.2008.03.182   (Journal Full-text)

PURPOSE: We investigated the effect of the duration of vaginal distention on the differential expression of stem cell homing, tissue repair cytokines and cytokine receptors to identify the factors most important for recovery from injury. MATERIALS AND METHODS: A total of 20, 10-week-old virgin Sprague-Dawley rats were divided into 4 groups, including 1, 4 and 6-hour vaginal distention, and anesthetized sham operation. The vagina, bladder, urethra and rectum were harvested immediately after vaginal distention. Real-time polymerase chain reaction was used to determine the relative expression of cytokines and receptors of interest. Mixed models analysis was used to determine associations between expression levels and vaginal distention duration. RESULTS: Positive associations between vaginal distention duration and the urethral expression level were found for 1 of the receptors of monocyte chemotactic protein-3, CCR1 (p = 0.0001) as well as for monocyte chemotactic protein-3 (p = 0.025), CCR5 (p = 0.032) and hypoxia inducible factor-1alpha (p = 0.023). A positive relationship between vaginal distention duration and monocyte chemotactic protein-3 expression was also observed in rectal tissue (p = 0.035). Urethral expression of CCR2, another receptor for monocyte chemotactic protein-3, approached significance (p = 0.066). An inverse relationship between vaginal distention duration and interleukin-8 expression was found in the bladder (p = 0.0008). No association was noted between vaginal distention duration and the expression of stromal derived factor-1, CXCR4, CCR3 and vascular endothelial growth factor in any pelvic organs. CONCLUSIONS: These data support a relationship between vaginal distention duration and the subsequent expression of monocyte chemotactic protein-3 and 1 of its associated receptors, CCR1, in the urethra immediately following vaginal distention. The increase in hypoxia-inducible factor1alpha expression in the urethra with prolonged vaginal distention suggests a limited role of tissue ischemia in the immediate response of pelvic organs to vaginal distention.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Birth Injuries  ISOCcr5 (Rattus norvegicus)4890426; 4890426mRNA:increased expression:multiple organs (rat)RGD 
Birth Injuries  IEP 4890426mRNA:increased expression:multiple organs (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ccr5  (C-C motif chemokine receptor 5)

Genes (Mus musculus)
Ccr5  (C-C motif chemokine receptor 5)

Genes (Homo sapiens)
CCR5  (C-C motif chemokine receptor 5)


Additional Information