RGD Reference Report - Dynamic expression of tenascin-C after myocardial ischemia and reperfusion: assessment by 125I-anti-tenascin-C antibody imaging. - Rat Genome Database

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Dynamic expression of tenascin-C after myocardial ischemia and reperfusion: assessment by 125I-anti-tenascin-C antibody imaging.

Authors: Taki, J  Inaki, A  Wakabayashi, H  Imanaka-Yoshida, K  Ogawa, K  Hiroe, M  Shiba, K  Yoshida, T  Kinuya, S 
Citation: Taki J, etal., J Nucl Med. 2010 Jul;51(7):1116-22. Epub 2010 Jun 16.
RGD ID: 4889599
Pubmed: PMID:20554738   (View Abstract at PubMed)
DOI: DOI:10.2967/jnumed.109.071340   (Journal Full-text)

Tenascin-C, an extracellular matrix glycoprotein, appears only in the early stages of embryonic development. It is not normally expressed in the adult heart but does reappear transiently in distinct areas in association with active tissue remodeling. The aim of this study was to explore serial changes in the expression of tenascin-C after myocardial ischemia and reperfusion, using (125)I-labeled anti-tenascin-C antibody ((125)I-TNC-Ab) in a rat model of acute ischemia and reperfusion. METHODS: The left coronary artery was occluded for 20 or 30 min, followed by reperfusion for 1, 3, or 7 d in rats with 20 min of ischemia and for 1, 3, 7, 14, or 28 d in rats with 30 min of ischemia. At the time of the study, (125)I-TNC-Ab (1.0-2.5 MBq) was injected. Three to 5 h later, to verify the area at risk, (99m)Tc-methoxyisobutylisonitrile (100-200 MBq) was injected intravenously just after the left coronary artery reocclusion and the rats were sacrificed 1 min later. Dual-tracer autoradiography was performed to assess (125)I-TNC-Ab uptake and the area at risk. RESULTS: In rats with 20 min of ischemia, (125)I-TNC-Ab uptake peaked at 3 d after reperfusion, followed by faint uptake after 7 d (uptake ratios at 1, 3, and 7 d after reperfusion were 1.81 +/- 0.53, 2.46 +/- 0.79, and 1.23 +/- 0.17, respectively [P < 0.05 vs. 3 d]). In rats with 30 min of ischemia, uptake was high at 1 and 3 d after reperfusion (2.99 +/- 0.90 and 2.71 +/- 0.80, respectively), decreased at 7 and 14 d (1.94 +/- 0.23 and 2.06 +/- 0.37, respectively), and was weak at 28 d (1.47 +/- 0.27, P < 0.005 vs. 1 d, P < 0.05 vs. 3 d). CONCLUSION: These data indicate that (125)I-TNC-Ab imaging may be a way to monitor myocardial injury and its repair process after ischemia and reperfusion by visualizing tenascin-C expression.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Myocardial Reperfusion Injury  ISOTnc (Rattus norvegicus)4889599; 4889599 RGD 
Myocardial Reperfusion Injury  IEP 4889599 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Tnc  (tenascin C)

Genes (Mus musculus)
Tnc  (tenascin C)

Genes (Homo sapiens)
TNC  (tenascin C)


Additional Information