RGD Reference Report - Switching of platelet-activating factor acetylhydrolase catalytic subunits in developing rat brain. - Rat Genome Database

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Switching of platelet-activating factor acetylhydrolase catalytic subunits in developing rat brain.

Authors: Manya, H  Aoki, J  Watanabe, M  Adachi, T  Asou, H  Inoue, Y  Arai, H  Inoue, K 
Citation: Manya H, etal., J Biol Chem. 1998 Jul 17;273(29):18567-72.
RGD ID: 4107046
Pubmed: PMID:9660828   (View Abstract at PubMed)

In a previous study, we demonstrated that Platelet-activating Factor (PAF) acetylhydrolase purified from bovine brain cortical cytosol consists of two mutually homologous catalytic subunits (alpha1 and alpha2) and one putative regulatory beta subunit. The latter is a product of the LIS1 gene, which is defective in the Miller-Dieker syndrome, a form of lissencephaly. In this study, we examined the expression patterns of these three subunits in the developing rat brain. All three subunits were expressed in embryonic brain, whereas only alpha2 and beta subunit were detected in the adult brain by Western blotting. Biochemical analyses revealed that the alpha1/alpha2 heterodimer and alpha2/alpha2 homodimer are major catalytic units of embryonic and adult brain PAF acetylhydrolases, respectively. The alpha1 transcript and protein were detected predominantly in embryonic and postnatal neural tissues, such as the brain and spinal cord. Furthermore, we found using primary cultured cells isolated from neonatal rat brain that alpha1 protein were expressed only in neurons but not in glial cells and fibroblasts. In contrast, alpha2 and beta transcripts and proteins were detected both in neural and non-neural tissues, and their expression level was almost constant from fetal stages through adulthood. These results indicate that alpha1 expression is restricted to actively migrating neurons in rats and that switching of catalytic subunits from the alpha1/alpha2 heterodimer to the alpha2/alpha2 homodimer occurred in these cells during brain development, suggesting that PAF acetylhydrolase plays a role(s) in neuronal migration.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
1-alkyl-2-acetylglycerophosphocholine esterase complex part_ofIDA 4107046; 4107046; 4107046PMID:9660828UniProt 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Pafah1b1  (platelet-activating factor acetylhydrolase 1b, regulatory subunit 1)
Pafah1b2  (platelet-activating factor acetylhydrolase 1b, catalytic subunit 2)
Pafah1b3  (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3)


Additional Information