RGD Reference Report - Detection of α-fetoprotein and glypican-3 mRNAs in the peripheral blood of hepatocellular carcinoma patients by using multiple FQ-RT-PCR. - Rat Genome Database

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Detection of α-fetoprotein and glypican-3 mRNAs in the peripheral blood of hepatocellular carcinoma patients by using multiple FQ-RT-PCR.

Authors: Yan, Dong  He, Qingfang  Chen, Yaping  Wang, Lixin  Zhang, Xinwei 
Citation: Yan D, etal., J Clin Lab Anal. 2011;25(2):113-7. doi: 10.1002/jcla.20443.
RGD ID: 243065125
Pubmed: PMID:21438004   (View Abstract at PubMed)
PMCID: PMC6647726   (View Article at PubMed Central)
DOI: DOI:10.1002/jcla.20443   (Journal Full-text)

This work aimed to investigate the correlation of the expression of α-fetoprotein (AFP) and glypican-3 (GPC3) mRNAs in the peripheral blood with primary hepatocellular carcinoma (HCC) and HCC metastasis by using multiple fluorescence quantitative reverse transcriptase-polymerase chain reaction (FQ-RT-PCR). Peripheral blood samples from 100 patients with HCC were collected. The positive expression rates of AFP mRNA of HCC, hepatitis B, and cirrhosis patients were 56, 5, and 10%, respectively. AFP mRNA was not detected in healthy subjects, hepatic hemangioma, or hepatic metastasis patients' samples. Those of GPC3 mRNA of HCC patients were 76%. GPC3 mRNA was not detected in healthy subjects, hepatitis B, cirrhosis, hepatic hemangioma, or hepatic metastasis patients' samples. In HCC patients' samples, the combined positive rate of AFP and GPC3 mRNA expressions was 81%. The relative expression levels of GPC3 mRNA in the metastasis group and nonmetastasis group were 0.98±0.38 and 0.72±0.26, respectively, and showed significantly different (P=0.001). However, no significant difference was observed in the AFP mRNA expression levels (P=0.134). In conclusion, the sensitivity of HCC diagnosis can be improved by combined detection of AFP and GPC3 mRNA expressions. GPC3 mRNA is HCC-specific, and may indicate HCC metastasis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
hepatocellular carcinoma disease_progressionIEP 243065125mRNA:increased expression:Peripheral Blood (human)RGD 
hepatocellular carcinoma disease_progressionISOGPC3 (Homo sapiens)243065125; 243065125mRNA:increased expression:Peripheral Blood (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gpc3  (glypican 3)

Genes (Mus musculus)
Gpc3  (glypican 3)

Genes (Homo sapiens)
GPC3  (glypican 3)


Additional Information