RGD Reference Report - Biochemical characterization of membrane-associated septin from rat brain. - Rat Genome Database

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Biochemical characterization of membrane-associated septin from rat brain.

Authors: Maimaitiyiming, M  Kumanogoh, H  Nakamura, S  Nagata, K  Suzaki, T  Maekawa, S 
Citation: Maimaitiyiming M, etal., J Neurochem. 2008 Aug;106(3):1175-83. Epub 2008 May 3.
RGD ID: 2317328
Pubmed: PMID:18466330   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1471-4159.2008.05450.x   (Journal Full-text)

Within the cell membrane there exist various microdomains (lipid rafts) in which specific lipids and proteins are assembled and these microdomains are recovered in the detergent-resistant low-density membrane fraction (DRM). Septin is a novel GTP-binding, cytoskeletal protein having various isoforms that assemble into homo- and heterooligomers and filaments. As the localization of septin 3 in DRM was found through a proteomics analysis of brain-derived DRM, the presence of other septin isoforms in DRM was studied. Western blotting analysis showed maturation-dependent enrichment of several septin isoforms in DRM prepared from synaptic plasma membrane (SPM). These isoforms were solubilized with high MgCl2 solution and recovered as the precipitate after dialysis to low ionic solution. Three times cycling of the extraction-dialysis process resulted in the partial purification of septin complex and electron microscopic observation of this fraction revealed rod-like structures in which building units were observed. The presence of heterooligomers was shown with western blotting after the separation of the MgCl2 extract with blue-native polyacrylamide gel electrophoresis. Immunoprecipitation assay using monoclonal anti-septin11 antibody also showed the presence of heterooligomers. These results show that septin localizes in the membrane microdomains of the SPM in adult brain and may have important roles in the membrane dynamics of neurons.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
presynaptic membrane  IDA 2317328 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Septin3  (septin 3)


Additional Information