RGD Reference Report - Regional distributions of choline acetyltransferase and acetylcholinesterase activities in layers of rat cerebellar vermis. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Regional distributions of choline acetyltransferase and acetylcholinesterase activities in layers of rat cerebellar vermis.

Authors: Ross, CD  Smith, JT  Godfrey, DA 
Citation: Ross CD, etal., J Histochem Cytochem. 1983 Jul;31(7):927-37.
RGD ID: 2301199
Pubmed: PMID:6854006   (View Abstract at PubMed)

Entire sagittal sections of rat cerebellar vermis were dissected into microgram-sized samples of molecular, granular, and white matter layers. Assayed activities of choline acetyltransferase and acetylcholinesterase were plotted back onto sectional maps of exact sample locations. On the average, the activities of choline acetyltransferase and acetylcholinesterase in the granular layer were about four and five times, respectively, those in the molecular layer. The highest activity of both enzymes was in the granular layer of the vestibulocerebellum, the nodulus and ventral uvula (lobules X and IXc of Larsell). This activity might be related to the secondary vestibulocerebellar projection, terminating as mossy fibers in the granular layer of this region. Intermediate levels of activity were found in the granular layer of the dorsal uvula (lobule IX, a and b). The lowest activities of both enzymes in the granular layer were in the culmen (lobule V). A 7.1-fold difference in choline acetyltransferase activity and a 4.5-fold difference in acetylcholinesterase activity were found between the granular layer of lobules V and X. The numerical density of aggregates of acetylcholinesterase staining product in the granular layer correlated much better with assayed acetylcholinesterase activity than with choline acetyltransferase activity.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
acetylcholine biosynthetic process  IDA 2301199 RGD 
acetylcholine catabolic process  IDA 2301199 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
acetylcholinesterase activity  IDA 2301199activity varied considerably between different layers of the cerebellar vermisRGD 
choline O-acetyltransferase activity  IDA 2301199activity varied considerably between different layers of the cerebellar vermisRGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
acetylcholine metabolic pathway  IDA 2301199; 2301199activities of Ache and Chat varied considerably between different layers of the cerebellar vermisRGD 
Objects Annotated

Genes (Rattus norvegicus)
Ache  (acetylcholinesterase)
Chat  (choline O-acetyltransferase)


Additional Information