RGD Reference Report - Gene expression profiling of ERBB receptors and ligands in human transitional cell carcinoma of the bladder. - Rat Genome Database

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Gene expression profiling of ERBB receptors and ligands in human transitional cell carcinoma of the bladder.

Authors: Amsellem-Ouazana, D  Bieche, I  Tozlu, S  Botto, H  Debre, B  Lidereau, R 
Citation: Amsellem-Ouazana D, etal., J Urol. 2006 Mar;175(3 Pt 1):1127-32.
RGD ID: 2289980
Pubmed: PMID:16469638   (View Abstract at PubMed)
DOI: DOI:10.1016/S0022-5347(05)00317-4   (Journal Full-text)

PURPOSE: The ErbB driven growth pathway has been implicated in most human epithelial malignancies. Therefore, its blockade is a promising therapeutic strategy and several candidate drugs are currently undergoing clinical trials. Paradoxically little is known of the expression pattern or clinical significance of the 4 ErbB receptors and their 11 ligands in TCC of the bladder. MATERIALS AND METHODS: To obtain further insight into the molecular pathogenesis of TCC we used quantitative real-time reverse transcriptase-polymerase chain reaction assay to quantify mRNA expression of the 4 ERBB and their 11 known ligand genes, including recently described EPGN/epigen, in 73 tumor samples. RESULTS: The level of mRNA of 4 ligand genes (EGF, NRG1, NRG2 and NRG3) was extremely low, that is detectable but not quantifiable. Six genes were over expressed (ERBB2, TGFA, HB-EGF, AREG, EREG and EPGN), 3 were under expressed (ERBB1, ERBB4 and NRG4) and 2 were over or under expressed (ERBB3 and BTC). ERBB2 and AREG expression differed between early stage tumors (pTa grade 1) and normal samples. The most marked differences in expression were ERBB3, EREG and NRG4 between superficial and muscle invasive tumors (p = 0.0069, 0.00007 and 0.0000001, respectively), and TGFA and NRG4 between low and high grade superficial tumors, and between pT1 or greater and pTa tumors. CONCLUSIONS: This study shows the involvement of the ERBB family and ligand genes in TCC. Most receptor and ligand genes are deregulated at different stages of carcinogenesis, implying that they should be studied simultaneously. Quantitative real-time reverse transcriptase-polymerase chain reaction could be used to determine ErbB signaling pathway status in individuals with a view to tailored therapy.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
transitional cell carcinoma  IEP 2289980mRNA:increased expression:urinary bladderRGD 
transitional cell carcinoma  ISOAREG (Homo sapiens)2289980; 2289980mRNA:increased expression:urinary bladderRGD 
transitional cell carcinoma  IEP 2289980; 2289980mRNA:decreased expression:urinary bladderRGD 
transitional cell carcinoma  IEP 2289980 RGD 
transitional cell carcinoma  ISOEGFR (Homo sapiens)2289980; 2289980mRNA:decreased expression:urinary bladderRGD 
transitional cell carcinoma  ISOERBB3 (Homo sapiens)2289980; 2289980 RGD 
transitional cell carcinoma  ISOERBB4 (Homo sapiens)2289980; 2289980mRNA:decreased expression:urinary bladderRGD 

Objects Annotated

Genes (Rattus norvegicus)
Areg  (amphiregulin)
Egfr  (epidermal growth factor receptor)
Erbb3  (erb-b2 receptor tyrosine kinase 3)
Erbb4  (erb-b2 receptor tyrosine kinase 4)

Genes (Mus musculus)
Areg  (amphiregulin)
Egfr  (epidermal growth factor receptor)
Erbb3  (erb-b2 receptor tyrosine kinase 3)
Erbb4  (erb-b2 receptor tyrosine kinase 4)

Genes (Homo sapiens)
AREG  (amphiregulin)
EGFR  (epidermal growth factor receptor)
ERBB3  (erb-b2 receptor tyrosine kinase 3)
ERBB4  (erb-b2 receptor tyrosine kinase 4)


Additional Information