RGD Reference Report - Congenic strains reveal the effect of the renin gene on skeletal muscle angiogenesis induced by electrical stimulation. - Rat Genome Database

RGD Reference Report - Congenic strains reveal the effect of the renin gene on skeletal muscle angiogenesis induced by electrical stimulation. - Rat Genome Database

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General

Congenic strains reveal the effect of the renin gene on skeletal muscle angiogenesis induced by electrical stimulation.
Authors:	De Resende, MM Amaral, SL Moreno, C Greene, AS
Citation:	de Resende MM, etal., Physiol Genomics. 2008 Jan 15;.
RGD ID:	2289092
Pubmed:	PMID:18198281 (View Abstract at PubMed)
DOI:	DOI:10.1152/physiolgenomics.00150.2007 (Journal Full-text)
Previous studies have indicated the importance of angiotensin II (ANGII) in skeletal muscle angiogenesis. The present study explored the effect of the regulation of the renin gene on angiogenesis induced by electrical stimulation, using physiological, pharmacological and genetic manipulations of the renin angiotensin system (RAS). Transfer of the entire chromosome 13, containing the physiologically regulated renin gene, from the normotensive inbred Brown Norway (BN) rat into the background of an inbred sub-strain of the Dahl salt-sensitive (SS/Mcwi) rat restored renin levels and the angiogenic response after electrical stimulation. This restored response was significantly attenuated when the SS-13BN/Mcwi consomic rats were treated with lisinopril or high salt diet. The role of ANGII on this effect was confirmed by the complete restoration of the skeletal muscle angiogenesis in SS/Mcwi rats infused with sub-pressor doses of ANGII. Congenic strains derived from the SS-13BN/Mcwi consomic were used to further verify the role of the renin gene in this response. Microvessel density was markedly increased after stimulation in congenic strains that contained the renin gene from the BN rat (congenic lines A and D). This angiogenic response was suppressed in control strains that carried regions of the BN genome just above (congenic line C) or just below the renin gene (congenic line B). The present study emphasizes the importance of maintaining normal renin regulation as well as ANGII levels during the angiogenesis process using a combination of physiological, genetic and pharmacological manipulation of the RAS. Key words: renin angiotensin system, chromosomal substitution, angiogenesis.

Phenotype Annotations Click to see Annotation Detail View

Mammalian Phenotype

Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
decreased mean systemic arterial blood pressure	induced	IAGP	controlled sodium content diet	2289092	compared to male SS/JrHsdMcwi	RGD
increased angiogenesis	induced	IAGP	common peroneal nerve direct electrical stimulation	2289092	compared to SS/JrHsdMcwi	RGD
increased angiogenesis	induced	IAGP	common peroneal nerve direct electrical stimulation	2289092; 2289092		RGD
increased circulating renin level		IAGP		2289092	compared to SS/JrHsdMcwi	RGD
increased renin activity		IAGP		2289092		RGD

Objects Annotated

Strains

SS-Chr 13^BN/Mcwi (NA)

SS.BN-(D13Hmgc64-RN34_13048990782)/Mcwi (NA)

SS.BN-(D13Rat20-D13Rat127)/Mcwi (NA)

Objects referenced in this article

Strain	SS.BN-(D13Rat20-D13Got22)/Mcwi	null	Rattus norvegicus
Strain	SS.BN-(D13Rat91-D13Rat179)/Mcwi	null	Rattus norvegicus
Strain	SS/JrHsdMcwi	null	Rattus norvegicus

Additional Information