RGD Reference Report - Expression of angiogenic factors is upregulated in DMBA-induced rat mammary pathologies. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Expression of angiogenic factors is upregulated in DMBA-induced rat mammary pathologies.

Authors: Yan, M  Schneider, J  Gear, R  Lu, F  LaDow, K  Warshawsky, D  Heffelfinger, SC 
Citation: Yan M, etal., Pathobiology. 2004;71(5):253-60.
RGD ID: 1626167
Pubmed: PMID:15459484   (View Abstract at PubMed)
DOI: DOI:10.1159/000080059   (Journal Full-text)

OBJECTIVE: In the 7,12-dimethylbenz[a]anthracene (DMBA) model of rat mammary carcinogenesis, microvascular density and angiogenic potential increase with progression from normal to invasive disease, but the mechanisms involved are unknown. Using RT-PCR, we determined the expression of angiogenic regulators in DMBA-induced intraductal hyperplasia (IDP), carcinoma in situ (CIS), invasive tumors (INV), as well as normal tissue. METHODS: RT-PCR was performed on frozen tissue sections of each type of pathology for factors known to regulate angiogenesis in other systems. RESULTS: MMP-2, MMP-9, uPA, PAI-1, IGF-2, BFGF, VEGF, ANG-1, IRS-1, and TSP-1 were significantly (p < or =0.05) upregulated in CIS and INV, whereas TIMP-1, ANG-2, MASPIN, IGF1-R and HBEGF were unchanged. IGF-1 was uniquely elevated in IDP. SPARC was downregulated in CIS. Inhibition of IGF-1R by the tyrphostin, AG1024, blocked endothelial tubulogenesis in vitro, confirming that IGF-1 functions as a regulator of angiogenesis. CONCLUSIONS: These data support the involvement of specific angiogenic mediators in mammary tumor formation. Angiogenesis at different stages of tumorigenesis may be regulated by unique factors.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Experimental Mammary Neoplasms  ISOThbs1 (Rattus norvegicus)1626167; 1626167mRNA:increased expression:mammary glandRGD 
Experimental Mammary Neoplasms  IEP 1626167mRNA:increased expression:mammary glandRGD 
in situ carcinoma  ISOAngpt1 (Rattus norvegicus)1626167; 1626167associated with Mammary Neoplasms more ...RGD 
in situ carcinoma  IEP 1626167associated with Mammary Neoplasms more ...RGD 
Neoplasm Invasiveness  ISOAngpt1 (Rattus norvegicus)1626167; 1626167associated with Mammary Neoplasms more ...RGD 
Neoplasm Invasiveness  IEP 1626167associated with Mammary Neoplasms more ...RGD 

Objects Annotated

Genes (Rattus norvegicus)
Angpt1  (angiopoietin 1)
Thbs1  (thrombospondin 1)

Genes (Mus musculus)
Angpt1  (angiopoietin 1)
Thbs1  (thrombospondin 1)

Genes (Homo sapiens)
ANGPT1  (angiopoietin 1)
THBS1  (thrombospondin 1)


Additional Information