RGD Reference Report - Xuan Bi Tong Yu Fang Promotes Angiogenesis via VEGF-Notch1/Dll4 Pathway in Myocardial Ischemic Rats. - Rat Genome Database

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Xuan Bi Tong Yu Fang Promotes Angiogenesis via VEGF-Notch1/Dll4 Pathway in Myocardial Ischemic Rats.

Authors: Li, Shuangdi  Dong, Jingrong  Ta, Guang  Liu, Yinghui  Cui, Junfeng  Li, Xiaohui  Song, Jing  Liu, Aidong  Cheng, Guangyu 
Citation: Li S, etal., Evid Based Complement Alternat Med. 2020 Feb 5;2020:5041629. doi: 10.1155/2020/5041629. eCollection 2020.
RGD ID: 155641250
Pubmed: PMID:32089723   (View Abstract at PubMed)
PMCID: PMC7025065   (View Article at PubMed Central)
DOI: DOI:10.1155/2020/5041629   (Journal Full-text)


OBJECTIVE: To investigate the effect of Xuan Bi Tong Yu Fang (XBTYF) on angiogenesis via the vascular endothelial growth factor- (VEGF-) Notch1/delta-like 4 (Dll4) pathway. Materials and Methods. Sixty Sprague-Dawley rats were randomly divided into six groups: control, sham-operated, myocardial ischemia model, and XBTYF treatment at 3.2, 1.6, and 0.8 g/kg. Electrocardiography was performed to evaluate the successful establishment of the model. Hematoxylin-eosin staining and transmission electron microscopy were carried out to observe the morphology and mitochondrial structure in myocardial cells, respectively. TUNEL staining was performed to assess the degree of cell apoptosis. The expression of VEGF-A, Notch1, Dll4, Bcl2, Bax, caspase 3, caspase 9, and cytochrome-c (Cyt-c) was observed by western blot.
RESULTS: XBTYF inhibited changes to the morphology and mitochondrial structure in cardiomyocyte and reduced cell apoptosis. Compared with the model group, XBTYF at all doses (3.2, 1.6, and 0.8 g/kg) reduced the expression of Notch1, Dll4, Bax, caspase 3, caspase 9, and Cyt-c, whereas expression of VEGF-A and Bcl2 was increased.
CONCLUSION: XBTYF attenuated mitochondrial damage and cell apoptosis while promoting the angiogenesis of cardiomyocyte. The associated mechanism may be related to the VEGF-Notch1/Dll4 pathway.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Myocardial Ischemia treatmentISODll4 (Rattus norvegicus)155641250; 155641250 RGD 
Myocardial Ischemia treatmentIEP 155641250; 155641250 RGD 
Myocardial Ischemia treatmentISONotch1 (Rattus norvegicus)155641250; 155641250 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dll4  (delta like canonical Notch ligand 4)
Notch1  (notch receptor 1)

Genes (Mus musculus)
Dll4  (delta like canonical Notch ligand 4)
Notch1  (notch 1)

Genes (Homo sapiens)
DLL4  (delta like canonical Notch ligand 4)
NOTCH1  (notch receptor 1)


Additional Information