RGD Reference Report - Proteomic analysis of liver mitochondria from rats with nonalcoholic steatohepatitis. - Rat Genome Database

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Proteomic analysis of liver mitochondria from rats with nonalcoholic steatohepatitis.

Authors: Li, Lin  Lu, De-Zhao  Li, You-Ming  Zhang, Xue-Qun  Zhou, Xin-Xin  Jin, Xi 
Citation: Li L, etal., World J Gastroenterol. 2014 Apr 28;20(16):4778-86. doi: 10.3748/wjg.v20.i16.4778.
RGD ID: 14694831
Pubmed: PMID:24782632   (View Abstract at PubMed)
PMCID: PMC4000516   (View Article at PubMed Central)
DOI: DOI:10.3748/wjg.v20.i16.4778   (Journal Full-text)


AIM: To explore mitochondrial dysfunction in nonalcoholic steatohepatitis (NASH) by analyzing the proteome of liver mitochondria from a NASH model.
METHODS: The NASH rat model was established by feeding rats a fat-rich diet for 24 wk and was confirmed using hematoxylin and eosin staining of liver tissue and by changes in the levels of serum alanine transaminase, aspartate aminotransferase, triglyceride, total cholesterol and other markers. Liver mitochondria from each group were isolated using differential centrifugation. The mitochondrial samples were lyzed, purified and further analyzed using two-dimensional electrophoresis combined with matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry. Bioinformatic analyses of assigned gene ontology and biological pathway was used to study functional enrichments in the abundant proteomic data.
RESULTS: Eight up-regulated and sixteen down-regulated proteins were identified that showed greater than 1.5-fold differences between the controls and the NASH group. These dysregulated proteins were predicted to be involved in different metabolic processes including fatty acid β-oxidation processes, lipid metabolic processes, cell-cycle arrest, cell polarity maintenance, and adenosine triphosphate/sex hormone metabolic processes. Novel proteins that may be involved in NASH pathogenesis including the trifunctional enzyme Hadha, thyroxine, prohibitin, aldehyde dehydrogenase ALDH1L2, UDP-glucuronosyltransferase 2B31, and carbamoyl-phosphate synthase were identified using bioinformatics tools. The decreased expression of Hadha in NASH liver was verified by Western blotting, which was used as a complementary technique to confirm the proteomic results.
CONCLUSION: This novel report on the liver mitochondrial proteome of a NASH model may provide a reservoir of information on the pathogenesis and treatment of NASH.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
metabolic dysfunction-associated steatotic liver disease  ISOHadha (Rattus norvegicus)14694831; 14694831protein:decreased expression:liverRGD 
metabolic dysfunction-associated steatotic liver disease  IEP 14694831protein:decreased expression:liverRGD 

Objects Annotated

Genes (Rattus norvegicus)
Hadha  (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha)

Genes (Mus musculus)
Hadha  (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha)

Genes (Homo sapiens)
HADHA  (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha)


Additional Information