RGD Reference Report - Identification of Transcription Factor-Gene Regulatory Network in Acute Myocardial Infarction. - Rat Genome Database

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Identification of Transcription Factor-Gene Regulatory Network in Acute Myocardial Infarction.

Authors: Cao, Yuejuan  Li, Rongqing  Li, Yangchun  Zhang, Tao  Wu, Nan  Zhang, Jianyan  Guo, Zhaozeng 
Citation: Cao Y, etal., Heart Lung Circ. 2017 Apr;26(4):343-353. doi: 10.1016/j.hlc.2016.06.1209. Epub 2016 Jul 26.
RGD ID: 13524557
Pubmed: PMID:27746059   (View Abstract at PubMed)
DOI: DOI:10.1016/j.hlc.2016.06.1209   (Journal Full-text)


BACKGROUND: Acute myocardial infarction (AMI) is a common disease with serious mortality and morbidity, worldwide. The present study aimed to identify differentially expressed genes (DEGs) and construct a transcription factor-gene regulatory network to further study the early diagnosis of AMI.
METHODS: The integrated analysis of publicly available Gene Expression Omnibus datasets of AMI was performed. Differentially expressed genes were identified between AMI and normal blood samples. Gene Ontology enrichment analyses, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and the transcription factor-gene regulatory network were used to obtain insights into the functions of DEGs. Quantitative real-time polymerase chain reactions (qRT-PCR) were performed to validate the expression level of DEGs.
RESULTS: A total of 2,502 DEGs, including 917 up-regulated genes and 1,585 down-regulated genes, were identified between AMI and normal blood samples by integrating four expression profiles of AMI. Differentially expressed genes were significantly enriched in pathways including complement and coagulation cascades, Staphylococcus aureus infection, and cell adhesion molecules. Transcription factors were screened and performed to construct the regulatory network. The transcription factor-gene regulatory network consisted of 871 interactions between 80 transcription factors and 716 DEGs. ETS homologous factor (EHF) was one of transcription factors that had high connectivity with DEGs and regulated CACNB4 in the network. Verification by qRT-PCR revealed that EHF, KRT6A and DSG3 were significantly up-regulated, while CACNG4 was significantly down-regulated in AMI. Furthermore, CACNG6, CACNB4 and CLDN18 had a tendency to be down-regulated, and CALML3 had a tendency to be up-regulated in AMI.
CONCLUSIONS: The identification of important differentially expressed transcription factors and genes in the development of AMI would be the groundwork for the early diagnosis and early intervention of AMI.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
acute myocardial infarction  IEP 13524557mRNA:decreased expression:blood:RGD 
acute myocardial infarction  ISOCACNG4 (Homo sapiens)13524557; 13524557mRNA:decreased expression:blood:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cacng4  (calcium voltage-gated channel auxiliary subunit gamma 4)

Genes (Mus musculus)
Cacng4  (calcium channel, voltage-dependent, gamma subunit 4)

Genes (Homo sapiens)
CACNG4  (calcium voltage-gated channel auxiliary subunit gamma 4)


Additional Information