RGD Reference Report - Expression of membrane-bound NPP-type ecto-phosphodiesterases in rat podocytes cultured at normal and high glucose concentrations. - Rat Genome Database

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Expression of membrane-bound NPP-type ecto-phosphodiesterases in rat podocytes cultured at normal and high glucose concentrations.

Authors: Jankowski, Maciej  Piwkowska, Agnieszka  Rogacka, Dorota  Audzeyenka, Irena  Janaszak-Jasiecka, Anna  Angielski, Stefan 
Citation: Jankowski M, etal., Biochem Biophys Res Commun. 2011 Dec 9;416(1-2):64-9. doi: 10.1016/j.bbrc.2011.10.144. Epub 2011 Nov 6.
RGD ID: 13204715
Pubmed: PMID:22086174   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2011.10.144   (Journal Full-text)

The ecto-nucleotide pyrophosphatase/phosphodiesterase family (E-NPPs) contains two membrane-bound members: E-NPP1 and E-NPP3. These enzymes mediate hydrolysis of extracellular nucleotides and their abnormal expression may affect intracellular signal transduction pathways, leading to cellular dysfunction, e.g., insulin resistance. Podocytes are insulin-dependent glomerular epithelial cells that regulate the glomerular filtration rate. Pathology of podocytes is a hallmark of diabetic nephropathy. Here, we investigated the expressions of E-NPP1 and E-NPP3 and activity of E-NPP enzymes in rat podocytes cultured with 5mM (NG) or 30 mM glucose (HG). Insulin resistance was determined by measuring changes in [1,2-(3)H]-deoxy-D-glucose uptake in response to insulin. mRNAs of E-NPP1 and E-NPP3 were detected within podocytes. The E-NPP expressions were confirmed at the protein level using western blot and immunofluorescence techniques. At NG, insulin (300 nM, 3 min) increased glucose uptake 1.5-fold; however, this effect was abolished at HG. The protein expressions of E-NPP1 and E-NPP3 were not affected at HG. The E-NPP activities were 24.68±0.72 and 26.51±1.55 nmol/min/mg protein at NG and HG, respectively. In conclusion, ecto-nucleotide pyrophosphatase/phosphodiesterase 1 and 3 are expressed on podocytes, but changes in expression of these enzymes are most likely not involved in etiology of insulin resistance in podocytes.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cell body  IDA 13204715podocyteRGD 
cell projection  IDA 13204715podocyteRGD 

Objects Annotated

Genes (Rattus norvegicus)
Enpp1  (ectonucleotide pyrophosphatase/phosphodiesterase 1)


Additional Information