RGD Reference Report - The Ewing's sarcoma protein interacts with the Tudor domain of the survival motor neuron protein. - Rat Genome Database

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The Ewing's sarcoma protein interacts with the Tudor domain of the survival motor neuron protein.

Authors: Young, PJ  Francis, JW  Lince, D  Coon, K  Androphy, EJ  Lorson, CL 
Citation: Young PJ, etal., Brain Res Mol Brain Res 2003 Nov 6;119(1):37-49.
RGD ID: 1304456
Pubmed: PMID:14597228   (View Abstract at PubMed)

The survival motor neuron (SMN) gene is the spinal muscular atrophy (SMA) determining gene. Here we report that the SMN protein product interacts in vitro and in vivo with the arginine/glycine (RG)-rich RNA binding protein and transcription factor, Ewing's sarcoma (EWS). Recently, the SMN encoded Tudor domain (exon 3) and the YG-motifs (exon 6) have been shown to be involved in binding to RG-rich proteins. Here, we demonstrate that the Tudor domain encoded by SMN exon 3 is independently sufficient to mediate the interaction with EWS. Synthetic mutations within the Tudor domain, as well as a SMA patient-derived mutation within exon 3, reduced the levels of the SMN/EWS interaction. Carboxyl-terminal SMN mutations that prevent formation of SMN oligomers also indirectly reduced EWS binding. A role for arginine methylation has been observed in some RG-containing SMN-interacting proteins. Here we demonstrate that SMN interacts with non-methylated EWS and an EWS-derived RG-containing peptide. In contrast to previously reported results, symmetrical dimethylation of the EWS-derived RG-peptide results in a quantitative increase in the dissociation rate between SMN and the symmetrical dimethylated EWS RG-peptide. Consistent with the interaction data, endogenous and transiently expressed SMN co-localizes with endogenous EWS in a number of cultured cell lines, as well as rat primary neuron cultures. Anti-sense RNA experiments, however, demonstrate that EWS does not mediate the nuclear distribution of SMN or other Cajal body components.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Cajal body  IDA 1304456 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ewsr1  (EWS RNA-binding protein 1)

Objects referenced in this article
Gene Smn1 survival of motor neuron 1, telomeric Rattus norvegicus

Additional Information