RGD Reference Report - Cast: a novel protein of the cytomatrix at the active zone of synapses that forms a ternary complex with RIM1 and munc13-1. - Rat Genome Database

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Cast: a novel protein of the cytomatrix at the active zone of synapses that forms a ternary complex with RIM1 and munc13-1.

Authors: Ohtsuka, T  Takao-Rikitsu, E  Inoue, E  Inoue, M  Takeuchi, M  Matsubara, K  Deguchi-Tawarada, M  Satoh, K  Morimoto, K  Nakanishi, H  Takai, Y 
Citation: Ohtsuka T, etal., J Cell Biol 2002 Aug 5;158(3):577-90. Epub 2002 Aug 5.
RGD ID: 1299401
Pubmed: PMID:12163476   (View Abstract at PubMed)
PMCID: PMC2173811   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.200202083   (Journal Full-text)

The cytomatrix at the active zone (CAZ) has been implicated in defining the site of Ca2+-dependent exocytosis of neurotransmitter. We have identified here a novel CAZ protein of approximately 120 kD from rat brain and named it CAST (CAZ-associated structural protein). CAST had no transmembrane segment, but had four coiled-coil domains and a putative COOH-terminal consensus motif for binding to PDZ domains. CAST was localized at the CAZ of conventional synapses of mouse brain. CAST bound directly RIM1 and indirectly Munc13-1, presumably through RIM1, forming a ternary complex. RIM1 and Munc13-1 are CAZ proteins implicated in Ca2+-dependent exocytosis of neurotansmitters. Bassoon, another CAZ protein, was also associated with this ternary complex. These results suggest that a network of protein-protein interactions among the CAZ proteins exists at the CAZ. At the early stages of synapse formation, CAST was expressed and partly colocalized with bassoon in the axon shaft and the growth cone. The vesicles immunoisolated by antibassoon antibody-coupled beads contained not only bassoon but also CAST and RIM1. These results suggest that these CAZ proteins are at least partly transported on the same vesicles during synapse formation.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
synapse assembly involved_inNAS 1299401PMID:12163476UniProt 
synapse organization  IEP 1299401 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
growth cone located_inIDA 1299401PMID:12163476UniProt 
neuronal cell body  IDA 1299401 RGD 
presynaptic membrane located_inIDA 1299401PMID:12163476UniProt 
protein-containing complex  IDA 1299401 RGD 
synapse located_inTAS 1299401PMID:12163476UniProt 
terminal bouton  IDA 1299401 RGD 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Bsn  (bassoon (presynaptic cytomatrix protein))
Erc2  (ELKS/RAB6-interacting/CAST family member 2)
Rims1  (regulating synaptic membrane exocytosis 1)


Additional Information