RGD Reference Report - Cluster analysis of mRNA expression levels identifies multiple sequential patterns following focal cerebral ischemia. - Rat Genome Database

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Cluster analysis of mRNA expression levels identifies multiple sequential patterns following focal cerebral ischemia.

Authors: Kaido, T  Kempski, O  Heimann, A  Heers, C  Bartsch, D 
Citation: Kaido T, etal., Turk Neurosurg. 2012;22(4):441-7. doi: 10.5137/1019-5149.JTN.5523-11.0.
RGD ID: 10053716
Pubmed: PMID:22843461   (View Abstract at PubMed)
DOI: DOI:10.5137/1019-5149.JTN.5523-11.0   (Journal Full-text)

AIM: The purpose of this study is to detect gene expression patterns following focal cerebral ischemia. MATERIAL AND METHODS: 25 male Wistar rats were divided into control (n = 8) and ischemic (n = 17) groups. In the ischemic group, slowly progressing focal ischemia was simulated by two-vein occlusion with spreading depression (SD) a cortical microinjection of KCl induced. Ischemic tissue was removed at 2, 8, 24, or 72 h postischemia. Using semiquantitative reverse transcription polymerase chain reaction, we investigated mRNA expression levels of 13 representative genes related to cerebral ischemia. Cluster analysis of the gene expression levels was done. RESULTS: In the ischemic group, the expression levels of c-fos, cyclin D1, and COX-2 were significantly higher at 2 h postischemia, and those of bcl-2, bcl-xL, and HO-1 at 72 h. Based on the cluster analysis, we statistically divided examined genes into three groups: group A, early expression (COX-2, c-fos, and bcl-2); group B, s expression (c-jun, SOD-1, bad, p53, SOD-2, bcl-xL, and bax); and group C, late expression (cyclin D1, c-myc, and HO-1). CONCLUSION: We statistically classified the genes into three groups after focal ischemia. The genes of the early- and late-expression groups can be possible therapeutic targets for the treatment of cerebral ischemia.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
brain ischemia  ISOBad (Rattus norvegicus)10053716; 10053716 RGD 
brain ischemia  ISOBcl2l1 (Rattus norvegicus)10053716; 10053716 RGD 
brain ischemia  IEP 10053716; 10053716 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Bad  (BCL2-associated agonist of cell death)
Bcl2l1  (Bcl2-like 1)

Genes (Mus musculus)
Bad  (BCL2-associated agonist of cell death)
Bcl2l1  (BCL2-like 1)

Genes (Homo sapiens)
BAD  (BCL2 associated agonist of cell death)
BCL2L1  (BCL2 like 1)


Additional Information