This strain was established by a use of the BAC transgenic method with a technical support from the C.B.S.N resource (Dr. Kazuto Kobayashi, Fukushima Medical University, Dr. Yuchio Yanagawa, Gunma University Graduate School of Medicine). This transgenic rat was transported to The Jikei University Sc
hool of Medicine, and cre recombinase expression was confirmed by crossing it with a reporter rat (NBRP Rat No: 0734). These rats are maintained in The Jikei University School of Medicine. Background strain: Crlj:WI.
Transgene: Dbh (dopamine beta-hydroxylase: rat), TetR (E.coli), VP16 protein minimal“F”-type activation domain (Herpes simplex virus), 2A (Thosea asigna virus), cre recombinase (P1 Phage), bGlobin polyA (rabbit), loxP (P1 phage). Detailed method (BAC transgenic method): a rat BAC clone was used (CH230-211J22). Combined mRNA for tTA and cre recombinase sequences is transcribed under the control of Dbh promoter. In translation step, the transcript is cut at the 2A peptide sequence, resulting in a separate protein production of tTA and Cre.
This strain was established by a use of the BAC transgenic method with a technical support from the C.B.S.N resource (Dr. Kazuto Kobayashi, Fukushima Medical University, Dr. Yuchio Yanagawa, Gunma University Graduate School of Medicine). This transgenic rat was transported to The Jikei University Sc
hool of Medicine, and cre recombinase expression was confirmed by crossing it with a reporter rat (NBRP Rat No: 0734). These rats are maintained in The Jikei University School of Medicine. Background strain: Crlj:WI.
Transgene: Dbh (dopamine beta-hydroxylase: rat), TetR (E.coli), VP16 protein minimal“F”-type activation domain (Herpes simplex virus), 2A (Thosea asigna virus), cre recombinase (P1 Phage), bGlobin polyA (rabbit), loxP (P1 phage). Detailed method (BAC transgenic method): a rat BAC clone was used (CH230-211J22). Combined mRNA for tTA and cre recombinase sequences is transcribed under the control of Dbh promoter. In translation step, the transcript is cut at the 2A peptide sequence, resulting in a separate protein production of tTA and Cre.
This strain was established by a use of the BAC transgenic method with a technical support from the C.B.S.N resource (Dr. Kazuto Kobayashi, Fukushima Medical University, Dr. Yuchio Yanagawa, Gunma University Graduate School of Medicine). This transgenic rat was transported to The Jikei University Sc
hool of Medicine, and cre recombinase expression was confirmed by crossing it with a reporter rat (NBRP Rat No: 0734). These rats are maintained in The Jikei University School of Medicine. Background strain: Crlj:WI.
Transgene: Dbh (dopamine beta-hydroxylase: rat), TetR (E.coli), VP16 protein minimal“F”-type activation domain (Herpes simplex virus), 2A (Thosea asigna virus), cre recombinase (P1 Phage), bGlobin polyA (rabbit), loxP (P1 phage). Detailed method (BAC transgenic method): a rat BAC clone was used (CH230-211J22). Combined mRNA for tTA and cre recombinase sequences is transcribed under the control of Dbh promoter. In translation step, the transcript is cut at the 2A peptide sequence, resulting in a separate protein production of tTA and Cre.
This congenic strain was created by back crossing Long-Evans with The W-Tg(Dbh-tTA-2A-cre)2_7Fusa strain(RGD: 598092605) provided by Dr. Fusao Kato and Dr. Yukari Takahashi of the Jikei University School of Medicine. Background strain: Long-Evans (Institute for