Gpr18 Reference Search Result - Rat Genome Database

Submit Data | Help | Video Tutorials | News | Publications | Download | REST API | Citing RGD | Contact

Advanced Search (OLGA)

References search result for All species
(View Results for all Objects and Ontologies)

2 records found for search term Gpr18

Refine Term:

Sort By:

All

Export

RGD ID	Title	Citation	Abstract	PubMed	Pub Date
11052895	GPR18 is required for a normal CD8alphaalpha intestinal intraepithelial lymphocyte compartment.	Wang X, etal., J Exp Med. 2014 Nov 17;211(12):2351-9. doi: 10.1084/jem.20140646. Epub 2014 Oct 27.	Intraepithelial lymphocytes (IELs) play an important role in maintaining the physiology of the small intestine. The majority of mouse IELs express CD8alphaalpha and are either gammadelta or alphabeta T cells. Although the development and homing of CD8alphaalpha IELs have been studied in some detail, ... (more) the factors controlling their homeostasis and positioning are incompletely understood. Here we demonstrate that G protein-coupled receptor 18 (GPR18) is abundantly expressed in CD8alphaalpha IELs and that mice lacking this orphan receptor have reduced numbers of gammadeltaT IELs. Mixed bone marrow chimera experiments reveal a markedly reduced contribution of GPR18-deficient cells to the CD8alphaalpha IEL compartment and a reduction in the CD8alphabeta T cell subset. These defects could be rescued by transduction with a GPR18-expressing retrovirus. The GPR18-deficient gammadeltaT IELs that remained in mixed chimeras had elevated Thy1, and there were less granzyme B(+) and Vgamma7(+) cells, indicating a greater reduction in effector-type cells. Flow cytometric analysis indicated GPR18 deficiency more strongly affected the CD8alphaalpha cells in the intraepithelial compared with the adjacent lamina propria compartment. These findings establish a requirement for GPR18 in CD8alphaalpha and CD8alphabeta IELs, and we suggest the receptor has a role in augmenting the accumulation of CD8 T cells in the intraepithelial versus lamina propria compartment.	25348153	2014-04-01
11076227	GPR18 Controls Reconstitution of Mouse Small Intestine Intraepithelial Lymphocytes following Bone Marrow Transplantation.	Becker AM, etal., PLoS One. 2015 Jul 21;10(7):e0133854. doi: 10.1371/journal.pone.0133854. eCollection 2015.	Specific G protein coupled receptors (GPRs) regulate the proper positioning, function, and development of immune lineage subsets. Here, we demonstrate that GPR18 regulates the reconstitution of intraepithelial lymphocytes (IELs) of the small intestine following ... (more) bone marrow transplantation. Through analysis of transcriptional microarray data, we find that GPR18 is highly expressed in IELs, lymphoid progenitors, and mature follicular B cells. To establish the physiological role of this largely uncharacterized GPR, we generated Gpr18-/- mice. Despite high levels of GPR18 expression in specific hematopoietic progenitors, Gpr18-/- mice have no defects in lymphopoiesis or myelopoiesis. Moreover, antibody responses following immunization with hapten-protein conjugates or infection with West Nile virus are normal in Gpr18-/- mice. Steady-state numbers of IELs are also normal in Gpr18-/- mice. However, competitive bone marrow reconstitution experiments demonstrate that GPR18 is cell-intrinsically required for the optimal restoration of small intestine TCRgammadelta+ and TCRalphabeta+ CD8alphaalpha+ IELs. In contrast, GPR18 is dispensable for the reconstitution of large intestine IELs. Moreover, Gpr18-/- bone marrow reconstitutes small intestine IELs similarly to controls in athymic recipients. Gpr18-/- chimeras show no changes in susceptibility to intestinal insults such as Citrobacter rodentium infections or graft versus host disease. These data reveal highly specific requirements for GPR18 in the development and reconstitution of thymus-derived intestinal IEL subsets in the steady-state and after bone marrow transplantation.	26197390	1000-05-01