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Strain Registration

Strain: SS-Vwfem4Mcwi

Symbol: SS-Vwfem4Mcwi
Strain: SS-Vwfem4
Substrain: Mcwi
RGD ID: 150429598
Citation ID: RRID:RGD_150429598
Ontology ID: RS:0005043
Alleles: Vwfem4Mcwi
Also Known As: SS-Vwf^[em4Mcwi]
Type: mutant
Available Source: Rat Genetic Models, through Versiti Blood Research Institute
Origination: Rat Genetic Models, through Versiti Blood Research Institute
Description: The rat strain was created via CRISPR/Cas9 targeting the VWF gene in DahlSS/Mcw (SS/JrHsdMcwi ) rat embryos. The resulting rat strain has a 13bp deletion in the untranslated region of Exon 52 of the VWF gene (g.158491511 - 158491523 on chromosome 4, Assembly: mRatBN7.2) The 13-bp deletion happens to be in the region where the polyadenylation signal resides (AAUAAA). The resulting mRNA is not polyadenylated and has trouble with transport from the nucleus to the cytoplasm. The result is a phenotype that is similar to a Type I von Willebrand Disease, being a partial quantitative deficiency of the circulating VWF protein. Some mRNA must make it through to translation, because low levels of VWF protein are detectable via ELISA (<10%). Both homozygous pairs and heterozygous pairs were used for breeding.
Last Known Status: Unknown
Research Usage Type I von Willebrand Disease
Position
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.24158,360,152 - 158,491,539RGD_MAPPER_PIPELINEmRatBN7.2
Rnor_6.04158,085,059 - 158,219,525RGD_MAPPER_PIPELINERnor6.0
Rnor_5.04225,098,521 - 225,229,257RGD_MAPPER_PIPELINERnor5.0
RGSC_v3.44161,723,415 - 161,854,766RGD_MAPPER_PIPELINERGSC3.4






References

References - curated
# Reference Title Reference Citation
1. Strains registered by the Montgomery Lab Strains registered from the Montgomery Lab from Versiti Blood Research Institute.

Region


Additional Information