RGD Reference Report - Different alterations in rat intestinal glutamine transport during the progression of CLP- and LPS-induced sepsis. - Rat Genome Database

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Different alterations in rat intestinal glutamine transport during the progression of CLP- and LPS-induced sepsis.

Authors: Niu, L  Qiao, W  Li, G  Li, Q  Huang, Q  Gong, J  Zhu, W  Li, N  Li, J 
Citation: Niu L, etal., J Surg Res. 2011 Aug;169(2):284-91. doi: 10.1016/j.jss.2009.11.732. Epub 2009 Dec 21.
RGD ID: 9999218
Pubmed: PMID:20338592   (View Abstract at PubMed)
DOI: DOI:10.1016/j.jss.2009.11.732   (Journal Full-text)

BACKGROUND: A marked deficiency of glutamine in clinical critical illness is correlated with mortality in the intensive care unit. Though intestinal glutamine transport was reported to be impaired in late sepsis, we hypothesized that there might be a different alteration in the early stage, with differential effects on the Na(+)-dependent glutamine transporters B(0)AT1, ATB(0,+), and ATA2. MATERIALS AND METHODS: Sepsis was induced by cecal ligation and puncture or lipopolysaccharide intraperitoneal injection in Sprague Dawley rats, and the samples were collected at 0, 2, 6, 12, 24h. Small intestinal brush border glutamine transport was studied by a rapid filtration technique. The relative contributions of the three main transporter, B(0)AT1, ATB(0,+), and ATA2, were determined by competitive inhibition. The mRNA level of each transporter was analyzed by RT-PCR, and an extra immunohistochemistry analysis was performed to detect the localization of ATA2 protein in small intestine. Serum TNF-alpha and IL-10 concentrations were quantitated by ELISA. RESULTS: Intestinal glutamine transport showed a biphasic change with an early increase and a late decrease in both CLP and LPS group. The early increase of glutamine transport was mainly attributable to the increased contributions of ATA2 and ATB(0,+). The transport activities of B(0)AT1, ATB(0,+) altered mainly because of the number of transporters (mRNA level as an indicator), while turned to ATA2, the redistribution was also found to be involved. The plasma TNF-alpha and IL-10 levels, especially the former, showed similar changing profiles to glutamine transport and, thus, may have relevance to it. CONCLUSION: Rat intestinal glutamine transport showed an early increase and a late decrease in sepsis, and may provide some information for sepsis treatment.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SLC38A2HumanSepsis  ISOSlc38a2 (Rattus norvegicus) RGD 
Slc38a2RatSepsis  IEP  RGD 
Slc38a2MouseSepsis  ISOSlc38a2 (Rattus norvegicus) RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc38a2Ratbrush border  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc38a2  (solute carrier family 38, member 2)

Genes (Mus musculus)
Slc38a2  (solute carrier family 38, member 2)

Genes (Homo sapiens)
SLC38A2  (solute carrier family 38 member 2)


Additional Information