RGD Reference Report - Characterization of 2-enoyl thioester reductase from mammals. An ortholog of YBR026p/MRF1'p of the yeast mitochondrial fatty acid synthesis type II. - Rat Genome Database

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Characterization of 2-enoyl thioester reductase from mammals. An ortholog of YBR026p/MRF1'p of the yeast mitochondrial fatty acid synthesis type II.

Authors: Miinalainen, IJ  Chen, ZJ  Torkko, JM  Pirila, PL  Sormunen, RT  Bergmann, U  Qin, YM  Hiltunen, JK 
Citation: Miinalainen IJ, etal., J Biol Chem. 2003 May 30;278(22):20154-61. Epub 2003 Mar 24.
RGD ID: 8553564
Pubmed: PMID:12654921   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M302851200   (Journal Full-text)

A data base search with YBR026c/MRF1', which encodes trans-2-enoyl thioester reductase of the intramitochondrial fatty acid synthesis (FAS) type II in yeast (Torkko, J. M., Koivuranta, K. T., Miinalainen, I. J., Yagi, A. I., Schmitz, W., Kastaniotis, A. J., Airenne, T. T., Gurvitz, A., and Hiltunen, K. J. (2001) Mol. Cell. Biol. 21, 6243-6253), revealed the clone AA393871 (HsNrbf-1, nuclear receptor binding factor 1) in human EST data bank. Expression of HsNrbf-1, tagged C-terminally with green fluorescent protein, in HeLa cells, resulted in a punctated fluorescence signal, superimposable with the MitoTracker Red dye. Wild-type polypeptide was immunoisolated from the extract of bovine heart mitochondria. Recombinant HsNrbf-1p reduces trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH-dependent manner with preference to medium chain length substrate. Furthermore, expression of HsNRBF-1 in the ybr026cDelta yeast strain restored mitochondrial respiratory function allowing growth on glycerol. These findings provide evidence that Nrbf-1ps act as a mitochondrial 2-enoyl thioester reductase, and mammalian cells may possess bacterial type fatty acid synthetase (FAS type II) in mitochondria, in addition to FAS type I in the cytoplasm.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MecrRatfatty acid metabolic process involved_inISSUniProtKB:Q9BV79PMID:12654921UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MecrRatmitochondrion located_inIDA PMID:12654921UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MecrRatenoyl-[acyl-carrier-protein] reductase (NADPH) activity enablesISSUniProtKB:Q9BV79PMID:12654921UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Mecr  (mitochondrial trans-2-enoyl-CoA reductase)


Additional Information