RGD Reference Report - Tpr is localized within the nuclear basket of the pore complex and has a role in nuclear protein export. - Rat Genome Database

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Tpr is localized within the nuclear basket of the pore complex and has a role in nuclear protein export.

Authors: Frosst, P  Guan, T  Subauste, C  Hahn, K  Gerace, L 
Citation: Frosst P, etal., J Cell Biol. 2002 Feb 18;156(4):617-30. Epub 2002 Feb 11.
RGD ID: 8553300
Pubmed: PMID:11839768   (View Abstract at PubMed)
PMCID: PMC2174070   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.200106046   (Journal Full-text)

Tpr is a coiled-coil protein found near the nucleoplasmic side of the pore complex. Since neither the precise localization of Tpr nor its functions are well defined, we generated antibodies to three regions of Tpr to clarify these issues. Using light and EM immunolocalization, we determined that mammalian Tpr is concentrated within the nuclear basket of the pore complex in a distribution similar to Nup153 and Nup98. Antibody localization together with imaging of GFP-Tpr in living cells revealed that Tpr is in discrete foci inside the nucleus similar to several other nucleoporins but is not present in intranuclear filamentous networks (Zimowska et al., 1997) or in long filaments extending from the pore complex (Cordes et al., 1997) as proposed. Injection of anti-Tpr antibodies into mitotic cells resulted in depletion of Tpr from the nuclear envelope without loss of other pore complex basket proteins. Whereas nuclear import mediated by a basic amino acid signal was unaffected, nuclear export mediated by a leucine-rich signal was retarded significantly. Nuclear injection of anti-Tpr antibodies in interphase cells similarly yielded inhibition of protein export but not import. These results indicate that Tpr is a nucleoporin of the nuclear basket with a role in nuclear protein export.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
TprRatnuclear pore complex assembly NOT|involved_inIMP PMID:11839768UniProt 
TprRatpositive regulation of protein export from nucleus involved_inIMP PMID:11839768UniProt 
TprRatprotein export from nucleus  IMP  RGD 
TprRatregulation of protein import into nucleus NOT|involved_inIMP PMID:11839768UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nup153Ratnuclear inclusion body located_inIDA PMID:11839768UniProt 
Nup98Ratnuclear inclusion body located_inIDA PMID:11839768UniProt 
TprRatnuclear inclusion body located_inIDA PMID:11839768UniProt 
Nup153Ratnuclear membrane located_inIDA PMID:11839768UniProt 
Nup98Ratnuclear membrane located_inIDA PMID:11839768UniProt 
TprRatnuclear membrane located_inIDA PMID:11839768UniProt 
Nup153Ratnuclear periphery located_inIDA PMID:11839768UniProt 
Nup98Ratnuclear periphery located_inIDA PMID:11839768UniProt 
TprRatnuclear periphery located_inIDA PMID:11839768UniProt 
Nup153Ratnuclear pore nuclear basket part_ofIDA PMID:11839768UniProt 
Nup98Ratnuclear pore nuclear basket part_ofIDA PMID:11839768UniProt 
TprRatnuclear pore nuclear basket part_ofIDA PMID:11839768UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Nup153  (nucleoporin 153)
Nup98  (nucleoporin 98 and 96 precursor)
Tpr  (translocated promoter region, nuclear basket protein)


Additional Information