RGD Reference Report - Interaction of the p62 subunit of dynactin with Arp1 and the cortical actin cytoskeleton. - Rat Genome Database

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Interaction of the p62 subunit of dynactin with Arp1 and the cortical actin cytoskeleton.

Authors: Garces, JA  Clark, IB  Meyer, DI  Vallee, RB 
Citation: Garces JA, etal., Curr Biol 1999 Dec 16-30;9(24):1497-500.
RGD ID: 70710
Pubmed: PMID:10607597   (View Abstract at PubMed)

Targeting of the minus-end directed microtubule motor cytoplasmic dynein to a wide array of intracellular substrates appears to be mediated by an accessory factor known as dynactin [1-4]. Dynactin is a multi-subunit complex that contains a short actin-related protein 1 (Arp 1) filament with capZ at the barbed end and p62 at the pointed end [5]. The location of the p62 subunit and the proposed role for dynactin as a multifunctional targeting complex raise the possibility of a dual role for p62 in dynein targeting and in Arp1 pointed-end capping. In order to gain further insight into the role of p62 in dynactin function, we have cloned cDNAs that encode two full-length isoforms of the protein from rat brain. We found that p62 is homologous to the nuclear migration protein Ropy-2 from Neurospora [6]; both proteins contain a zinc-binding motif that resembles the LIM domain of several other cytoskeletal proteins [7]. Overexpression of p62 in cultured mammalian cells revealed colocalization with cortical actin, stress fibers, and focal adhesion sites, sites of potential interaction between microtubules and the cell cortex [8,9]. The p62 protein also colocalized with polymers of overexpressed wild-type or barbed-end-mutant Arp1, but not with a pointed-end mutant. Deletion of the LIM domain abolished targeting of p62 to focal-adhesion sites but did not interfere with binding of p62 to actin or Arp1. These data implicate p62 in Arp1 pointed-end binding and suggest additional roles in linking dynein and dynactin to the cortical cytoskeleton.




Cellular Component

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Dctn4Ratfocal adhesion located_inIDA  RGD 
Dctn4Ratkinetochore  IDA  RGD 
Dctn4Ratspindle pole  IDA  RGD 
Dctn4Ratstress fiber located_inIDA  RGD 


Genes (Rattus norvegicus)
Dctn4  (dynactin subunit 4)