RGD Reference Report - Molecular cloning and nucleotide sequence of a cDNA encoding recoverin from human retina. - Rat Genome Database

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Molecular cloning and nucleotide sequence of a cDNA encoding recoverin from human retina.

Authors: Wiechmann, AF  Hammarback, JA 
Citation: Wiechmann AF and Hammarback JA, Exp Eye Res 1993 Apr;56(4):463-70.
RGD ID: 704325
Pubmed: PMID:8500558   (View Abstract at PubMed)
DOI: DOI:10.1006/exer.1993.1059   (Journal Full-text)

In the process of photoreceptor signal transduction, light initiates an enzymatic cascade that leads to hydrolysis of cyclic GMP (cGMP) and closure of cGMP-gated sodium-calcium channels resulting in photoreceptor hyperpolarization. Recoverin is a calcium-binding protein that is thought to reverse the effects of light on cGMP levels by activating guanylate cyclase. Guanylate cyclase produces cGMP to overcome the cGMP-hydrolysing effect of phosphodiesterase, and reopens the sodium-calcium channels in photoreceptor outer segments. We have cloned and sequenced a cDNA encoding recoverin in human retina. The human nucleotide sequence is 88% identical to the bovine sequence, and contains a 600-base pair (bp) open reading frame encoding 200 amino acids. In situ hybridization of cultured Y79 human retinoblastoma cells with a radioactive recoverin cDNA probe showed intense, specific labeling of the cytoplasm, indicating the presence of mRNA encoding recoverin. Direct sequencing of a Y79 retinoblastoma cDNA polymerase chain reaction (PCR) product confirmed the presence of recoverin in this human cell line.



Objects referenced in this article
Gene Rcvrn recoverin Rattus norvegicus

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