RGD Reference Report - An isoform of the Golgi t-SNARE, syntaxin 5, with an endoplasmic reticulum retrieval signal. - Rat Genome Database

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An isoform of the Golgi t-SNARE, syntaxin 5, with an endoplasmic reticulum retrieval signal.

Authors: Hui, N  Nakamura, N  Sonnichsen, B  Shima, DT  Nilsson, T  Warren, G 
Citation: Hui N, etal., Mol Biol Cell 1997 Sep;8(9):1777-87.
RGD ID: 68314
Pubmed: PMID:9307973   (View Abstract at PubMed)
PMCID: PMC305736   (View Article at PubMed Central)

The early Golgi t-SNARE (target-membrane-associated soluble-N-ethylmaleimide-sensitive factor attachment protein receptor) syntaxin 5 is thought to specify the docking site for both COPI and COPII coated vesicles originating from the endoplasmic reticulum (ER) and COPI vesicles on the retrograde pathway. We now show that there are two forms of syntaxin 5 that appear to be generated from the same mRNA by alternative initiation of translation. The short form (35 kDa) corresponds to the published sequence. The long form (42 kDa) has an N-terminal cytoplasmic extension containing a predicted type II ER retrieval signal. When grafted onto a reporter molecule, this signal localized the construct to the ER. Biochemical fractionation and immunofluorescence microscopy showed that there was less of the long form in the Golgi apparatus and more in peripheral punctate structures, some of which colocalized with markers of the intermediate compartment. The predicted absence of the long form in budding yeast points to a function unique to higher organisms.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Stx5RatGolgi membrane  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Stx5  (syntaxin 5)


Additional Information