RGD Reference Report - Structure of rat gamma-tubulin and its binding to HP33. - Rat Genome Database

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Structure of rat gamma-tubulin and its binding to HP33.

Authors: Nakadai, T  Okada, N  Makino, Y  Tamura, T 
Citation: Nakadai T, etal., DNA Res 1999 Jun 30;6(3):207-9.
RGD ID: 634415
Pubmed: PMID:10470852   (View Abstract at PubMed)

Gamma-tubulin is localized at the microtubule organizing center and is thought to participate in the organizing of the microtubule network. In this study, we isolated a cDNA of rat gamma-tubulin. The rat gamma-tubulin cDNA encoded 451 amino acids, the same number as that of its counterpart in other vertebrates, and its structure was found to be highly conserved in vertebrates. In a previous work, we identified HP33 (hepatocarcinogenesis- and hepatocellular proliferation-related 33-kDa protein) that was localized at the centrosome of hepatic cells and that exhibited MAP-like activity. In vitro GST pull-down assay using highly purified recombinant HP33 and bacterially expressed gamma-tubulin demonstrated that HP33 bound to gamma-tubulin directly. These results suggest that HP33 is localized at the centrosome via association with both the microtubule and its minus end-specific component, gamma-tubulin.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Tubg1Ratmicrotubule nucleation  TAS  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Glyatl2Ratcentrosome located_inNAS PMID:10470852UniProt 
Tubg1Ratgamma-tubulin complex  IDA  RGD 
Tubg1Ratmicrotubule organizing center  TAS  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Glyatl2Ratgamma-tubulin binding enablesNAS PMID:10470852UniProt 
Tubg1Ratprotein binding  IPIGlyatl2 (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Glyatl2  (glycine-N-acyltransferase-like 2)
Tubg1  (tubulin, gamma 1)


Additional Information