RGD Reference Report - A novel RNA binding protein, SBP2, is required for the translation of mammalian selenoprotein mRNAs. - Rat Genome Database

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A novel RNA binding protein, SBP2, is required for the translation of mammalian selenoprotein mRNAs.

Authors: Copeland, PR  Fletcher, JE  Carlson, BA  Hatfield, DL  Driscoll, DM 
Citation: Copeland PR, etal., EMBO J 2000 Jan 17;19(2):306-14.
RGD ID: 633967
Pubmed: PMID:10637234   (View Abstract at PubMed)
PMCID: PMC305564   (View Article at PubMed Central)
DOI: DOI:10.1093/emboj/19.2.306   (Journal Full-text)

In eukaryotes, the decoding of the UGA codon as selenocysteine (Sec) requires a Sec insertion sequence (SECIS) element in the 3' untranslated region of the mRNA. We purified a SECIS binding protein, SBP2, and obtained a cDNA clone that encodes this activity. SBP2 is a novel protein containing a putative RNA binding domain found in ribosomal proteins and a yeast suppressor of translation termination. By UV cross-linking and immunoprecipitation, we show that SBP2 specifically binds selenoprotein mRNAs both in vitro and in vivo. Using (75)Se-labeled Sec-tRNA(Sec), we developed an in vitro system for analyzing Sec incorporation in which the translation of a selenoprotein mRNA was both SBP2 and SECIS element dependent. Immunodepletion of SBP2 from the lysates abolished Sec insertion, which was restored when recombinant SBP2 was added to the reaction. These results establish that SBP2 is essential for the co-translational insertion of Sec into selenoproteins. We hypothesize that the binding activity of SBP2 may be involved in preventing termination at the UGA/Sec codon.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Secisbp2Ratselenocysteine incorporation involved_inIDA PMID:10637234UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Secisbp2RatRNA binding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Secisbp2  (SECIS binding protein 2)


Additional Information