RGD Reference Report - Murine cerebellar neurons express a novel gene encoding a protein related to cell cycle control and cell fate determination proteins. - Rat Genome Database

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Murine cerebellar neurons express a novel gene encoding a protein related to cell cycle control and cell fate determination proteins.

Authors: Taoka, M  Isobe, T  Okuyama, T  Watanabe, M  Kondo, H  Yamakawa, Y  Ozawa, F  Hishinuma, F  Kubota, M  Minegishi, A 
Citation: Taoka M, etal., J Biol Chem 1994 Apr 1;269(13):9946-51.
RGD ID: 632797
Pubmed: (View Article at PubMed) PMID:8144589

We cloned cDNAs of a novel protein (designated V-1) that has been identified from among the developmentally regulated proteins in the rat cerebellum. Protein sequencing analysis (Taoka, M., Yamakuni, T., Song, S.-Y., Yamakawa, Y., Seta, K., Okuyama, T., and Isobe, T. (1992) Eur. J. Biochem. 207, 615-620) and cDNA sequence analysis revealed that the V-1 protein consists of 117 amino acids and contains 2.5 contiguous repeats of the cdc10/SWI6 motif, which was originally found in the products of the cell cycle control genes of yeasts and the cell fate determination genes in Drosophila and Caenorhabditis elegans. In situ hybridization histochemistry revealed that the expression of the V-1 gene is transiently increased in postmigratory granule cells during postnatal rat cerebellar development and thereafter is markedly suppressed, whereas Purkinje cells constitutively express V-1 mRNA. In contrast, cerebellar granule cells of the staggerer mutant mouse continue to express the V-1 gene even when the granule cells of the normal mouse have ceased to express the V-1 gene, suggesting that the expression of the V-1 gene in granule cells is regulated through the interaction with Purkinje cells. On the basis of these results, we postulate that the V-1 protein has a potential role in the differentiation of granule cells.

Annotation

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Mtpn  (myotrophin)

Objects referenced in this article
Gene Mtpn myotrophin Mus musculus

Additional Information